大肠杆菌突变株QQS101发酵生产琥珀酸初步研究  

Succinate Production from Escherichia coli Mutant QQS101 Fermentation

作　　者：李宜奎[1,2] 康俊华[1] 康振[1] 耿艳平[1] 王义华[2] 祁庆生[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]江西农业大学理学院,南昌330045

出　　处：《中国生物工程杂志》2010年第10期39-43,共5页China Biotechnology

基　　金：国家"973"计划(2007CB707803);国家"863"计划(2006AA02Z218)资助项目

摘　　要：琥珀酸是一种具有重要应用价值的生物基平台化合物。对大肠杆菌focA-pflBldhA突变株QQS101在严格厌氧条件下生长和葡萄糖代谢能力进行了考察,比较分析了葡萄糖与大肠杆菌混合酸发酵产物的单位碳的还原程度,认为非严格厌氧条件有利于QQS101发酵葡萄糖积累琥珀酸,进一步对有氧生长碳源进行了对比试验的结果表明,以木糖支持有氧生长,QQS101摇瓶发酵39h消耗葡萄糖37.6g/L,琥珀酸的产量达到31.01g/L,摩尔产率为1.258molSuccinate/molGlucose。发酵过程中,丙氨酸的添加能够提高琥珀酸的摩尔产率。Succinate is an important bio-based platform molecule.In the present work,the growth and glucose metabolism of Escherichia coli mutant QQS101 with the deficiency of formate transporter A（focA）,formate-pyruvate lyase（pflB）and lactate dehydrogenase（ldhA）under strict anaerobic condition were investigated.The degree of reduction per carbon of glucose and the products of E.coli mix-acids fermentation were compared,and then recognized that non-strict anaerobic condition favored QQS101 producing succinate from glucose.Furthermore,effects of the carbon sources for aerobic growth on fermentation were performed.Results showed that QQS101 could accumulate succinate with a concentration of 31.01 g/L with a yield of 1.258 mol Succinate/mol Glucose,when growing on xylose as the aerobic substrate.During the fermentation,addition of alanine could enhance the molar yield of succinate to glucose utilized.

关 键 词：大肠杆菌 琥珀酸 木糖 丙氨酸 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]