急性胰腺炎肺损伤大鼠肺组织中Cav-1与水通道蛋白1、5的表达及清胰汤的治疗作用  

Expression of Cav-1, AQP1 and AQP5 in lung of acute pancreatitis-associated lung injury rats and the therapeutic role of Qingyitang

作　　者：王钢[2] 陈海龙[1] 任凤[3] 李雅琼[1] 李洁[1] 

机构地区：[1]大连医科大学第一附属医院普外科 ,116011 [2]国家医学考试中心 [3]大连医科大学基础医学院生物化学与分子生物学实验室

出　　处：《中华医学杂志》2010年第36期2564-2569,共6页National Medical Journal of China

基　　金：基金项目：国家自然科学基金（30973851、30770483）

摘　　要：目的 观察窖蛋白（Cav）1和水通道蛋白（AQP）1、5在急性胰腺炎肺损伤大鼠肺组织中的表达及定位,探讨其在急性胰腺炎肺损伤发病过程中的作用及清胰汤的可能作用机制.方法 将40只Wistar大鼠分为假手术组、模型组、地塞米松治疗组、清胰汤治疗组.经胰胆管逆行注射脱氧胆酸钠建立大鼠急性胰腺炎肺损伤模型.24 h后采血和肺组织,检测血清淀粉酶、肺湿/干重比值和肺组织病理切片判定损伤程度 RT-PCR检测肺组织中Cav-1和AQP1、AQP5的mRNA表达 提取脂筏,Western印迹检测Cav-1和AQP1、AQP5在脂筏内外的定位与表达.结果 模型组大鼠血清淀粉酶[（6152±502）U/L]、肺湿/干重比值（7.98±0.41）和肺组织病理损伤程度均明显升高.肺组织Cav-1和AQP1、AQP5均有表达,但在模型组mRNA表达水平均下调.Cav-1在脂筏内外均有表达,以脂筏内为主,AQP1完全定位在脂筏上,而AQP5则定位在脂筏外,三者在脂筏内外的分布状态没有改变,但表达量在模型组均明显下降.与模型组相比,地塞米松和清胰汤治疗组大鼠血清淀粉酶、肺湿/干重比值和肺组织病理损伤程度均下降 肺组织Cav-1和AQP1、AQP5的mRNA表达上调,在脂筏内外的表达水平有不同程度的恢复.结论 Cav-1和AQP1定位在脂筏,而AQP5定位在脂筏外,三者的表达下调与急性胰腺炎肺损伤密切相关.地塞米松和清胰汤都能够明显上调Cav-1和AQP1、AQP5的表达,有效减轻肺损伤程度.Objective To investigate the expression and location of caveolin-1 （Cav-1）, aquaporin 1 （ AQP1 ） and AQP5 in the lung of acute pancreatitis-associated lung injury rats and to determine the role of these molecules in the pathologic progress and the potential therapeutic mechanisms of Qingyitang. Methods Forty Wistar rats were randomly divided into sham operation （SHAM） group, acute lung injury （ALI）group, dexamethasone （DEX） group and Qingyitang （QYT） group. ALI was induced by reverse injection of deoxycholate into biliopancreatic duct of rats. Blood and lung tissues were collected after 24 h. Serum amylase, lung wet/dry （W/D） ratio and pathological section were detected to evaluate the degree of lung injury. reverse transcription-polymerase chain reaction was taken to detect the mRNA levels of Cav-1, AQP1 and AQP5. Lipid rafts were prepared for detection of the distribution and expression level of Cav-1, AQP1 and AQP5 proteins by Western blot. Results The concentration of serum amylase, the value of W/D and the degree of pathological lung injury obviously increased in ALI rats. Cav-1, AQP1 and AQP5 were present in the lung while the mRNA level decreased in ALI rats. Cav-1 appeared mainly in lipid rafts and less in non-lipid rafts. AQP1 was localized to lipid rafts while AQP5 to non-lipid rafts. The localization of these three molecules in the lung of ALI rats did not change compared with SHAM rats while their protein levels decreased. Compared with ALI rats, the concentration of serum amylase, the value of W/D and the degree of pathological lung injury obviously decreased in DEX and QYT rats. The mRNA and the protein expression of Cay-1, AQP1 and AQP5 increased in various degrees by DEX or QYT treatment. Conclusion Cav-1 and AQP1 are enriched in lipid rafts while AQP5 in non-lipid rafts. The down-regulated expression of these three molecules may play important role in acute pancreatitis-associated lung injury. DEX and QYT may relieve lung injury effectively by up-regulating the express

关 键 词：胰腺炎 急性坏死性 呼吸窘迫综合征 成人型 CAVEOLIN-1 水通道蛋白 

分 类 号：R686[医药卫生—骨科学]