粪肠球菌esp、gelE、ebpA基因及QS-fsr系统与生物被膜形成关系的研究  

Biofilm formation and esp, gelE, ebpA genes and QS-fsr system in Enterococcus faecalis

作　　者：漆涌[1] 邹彬彬[2] 伍勇[1] 

机构地区：[1]中南大学湘雅三医院检验科,长沙410013 [2]中南大学湘雅三医院长沙血液中心,长沙410013

出　　处：《中华检验医学杂志》2010年第10期931-935,共5页Chinese Journal of Laboratory Medicine

基　　金：国家质检总局科技计划项目（2008IK203）;湖南省自然科学基金资助项目（08JJ3069）

摘　　要：目的研究粪肠球菌esp、gelE、ebpA基因和QS-fsr系统与粪肠球菌生物被膜形成的相关性.方法收集2007年10月至2008年6月从湘雅三医院临床尿路感染患者尿液及导尿管中分离的粪肠球菌24株,按生物被膜阳性粪肠球菌及相应的生物被膜阴性粪肠球菌分为2组.使用RT-PCR分别对生物被膜阳性组和阴性组细菌的esp、ebpA、gelE和fsrB基因荧光强度比进行检测;利用real-time PCR分别检测生物被膜阳性组和阴性组细菌的esp、ebpA、gelE和fsrB 4种与生物被膜形成相关的基因的表达,并通过2-△△Ct方法进行相对定量.分析粪肠球菌esp、gelE、ebpA基因及QS-fsr系统与生物被膜形成的关系.结果 real-time PCR检测显示生物被膜阳性组粪肠球菌esp和ebpA表达量分别是生物被膜阴性组的298倍和59倍,生物被膜阳性组粪肠球菌gelE和fsrB表达量分别是生物被膜阴性组的1/244和1/249.生物被膜组和浮游生物组细菌esp、ebpA、gelE和fsB基因的荧光强度比,差异无统计学意义（秩和检验^值分别为92、79、42和34,P均＞0.05）.结论 esp、ebpA、gelE和fsrB基因与粪肠球菌生物被膜形成密切相关.esp和ebpA能促进生物被膜形成,gelE和fsB能抑制生物被膜形成.提示fsr系统能调节esp、ebpA和gelE基因的表达.Objective To investigate the association of esp, gelE, ebpA and QS-fsr system and biofilm formation in Enterococcus faecalis. Methods Totally 24 isolates of Enterococcus faecalis were collected from urine and catheter of clinical urine tract infection patients in Third Xiangya Hospital from Oct. 2007 to Jun. 2008, and were divided into biofilm group and non-biofilm group. The luminance ratios of esp, gelE, ebpA and fsrrB of Enterococcus faecalis in biofilm group and non-biofilm group were detected by RT-PCR. And the expression of esp, gelE, ebpA, fsrrB genes in different groups were detected by real-time PCR and were relatively quantitated through 2-△△Ct method. Moreover, the relevancies between that fourgenes and biofilm formation in Enterococcus faecalis were analyzed respectively. Results The expression of esp and ebpA in biofilm group were 298 times and 59 times more than the non-biofilm group. The expression level ofgelE and fsrB in biofilm group were 1/244 and 1/249 times less than the non-biofilm group, and the luminance ratios of esp, gelE, ebpA and fsrB were not significant between the two groups （rank sum was 92,79, 42 and 34 respectively,all P 〉 0. 05 ）. Conclusions The results showed that the biofilm formation in Enterococcus faecalis was promoted by esp and ebpA, and was inhabited by gelE and fsrB, which suggested that the expression of esp, ebpA and gelE genes was regulated by fsr system.

关 键 词：肠球菌 粪 细菌蛋白质类 纤毛 细菌 生物膜 泌尿道感染 

分 类 号：R686[医药卫生—骨科学]