骨髓基质细胞的体内成骨示踪研究  

Osteogenic and tracking study of bone marrow stromal stem cells in vivo

作　　者：陈瀛[1] 李子荣[1] 程立明[1] 洪闻[2] 闫然[2] 潘琳[3] 蔡哲 舒峻 

机构地区：[1]北京中日友好医院骨科,100029 [2]北京中日友好医院放射科,100029 [3]中日友好临床医学研究所

出　　处：《中华医学杂志》2010年第37期2652-2656,共5页National Medical Journal of China

基　　金：国家自然科学基金（30772194）

摘　　要：目的 探讨应用超顺磁性氧化铁（SPIO）纳米粒子标记骨髓基质细胞（MSCs）后自体移植,应用MRI进行体内示踪的可行性.方法 分离骨髓基质细胞,进行体外培养、SPIO标记,将标记细胞自体移植入动物股骨头内,进行活体核磁共振成像（MRI）检查及组织学对比检测.结果 SPIO标记细胞体内移植侧MRI检测可见明显的低信号改变.股骨头缺损内发现大量SPIO阳性细胞,很多骨陷窝及骨小梁表面细胞SPIO呈阳性.MSCs移植侧成骨程度强于对照侧.结论 MRI活体示踪可对MSCs移植后在活体内的迁徙情况提供影像学证据.移植的MSCs能在股骨头内成活,促进成骨.Objective To detect and track the presence of magnetically labeled bone marrow stromal cells （ MSCs ） in vivo with magnetic resonance imaging （ MRI ） after autologous transplantation.Methods The cultured and SPIO-labcled MSCs were transplanted into dog femoral head. The dog received regular MRI re-examination at post-operation. Samples were harvested at different weeks for analysis.Results The labeled cells showed an obviously low intensity of signal change in MRI. There were lots of labeled cells in the transplant area and more bone formation than the controls. Conclusion The labeled ceils can be visualized in vivo by MRI examination after transplantation into femoral head. The transplanted MSCs survive in vivo and increase bone formation.

关 键 词：股骨头坏死 骨髓 移植 自体 磁共振成像 

分 类 号：R686[医药卫生—骨科学]