致瘤素M对甲胎蛋白阳性人胚肝前体细胞体外分化的影响  

Effect of oncostatin M on differentiation of the AFP promoter-defined human hepatic progenitor cells in vitro

作　　者：刘保清[1] 李卫红[1] 张伟涛[1] 路欣[2] 安威[1] 张海燕[1] 

机构地区：[1]首都医科大学细胞生物学系"肝脏保护与再生调节"北京市重点实验室,北京100069 [2]首都医科大学人体解剖学与组织胚胎学系,北京100069

出　　处：《基础医学与临床》2010年第11期1153-1157,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30971472);北京市自然科学基金(5102013)

摘　　要：目的探讨致瘤素M(OSM)对甲胎蛋白阳性人胚肝前体细胞(APHPs)体外诱导分化的作用。方法相差显微镜观察APHPs的形态学;间接免疫荧光染色法、过碘酸希夫氏糖原染色法和透射电子显微镜技术检测APHPs中白蛋白(ALB)表达、糖原合成以及超微结构。结果 OSM作用3 d后,部分APHPs呈聚集生长,7 d后,APHPs形成"类组织样小结",具有较强的白蛋白表达和糖原合成能力,细胞内富含大量的线粒体、内质网及囊泡样结构,相邻细胞间存在明显的间隙,面向细胞间隙面的细胞表面形成大量的微绒毛结构。结论 OSM诱导APHPs形成的"类组织样小结"具有部分成熟肝实质细胞的功能。Objective To investigate the effect of oncostatin M（OSM） on differentiation of the α-fetoprotein（AFP） promoter-defined human hepatic progenitor cells（APHPs） in vitro.Methods The morphology of APHPs was observed by phase-contrast microscopy including the proliferation condition and OSM inducement.The properties of differentiated APHPs were detected after OSM treatment,which involved the albumin expression with immunofluorescence,the glycogen storage with periodic acid Schiff staining（PAS） and the subcellular structure with electron microcopy.Results Morphology of APHPs was changed after treatment with OSM,the APHPs were able to form cluster from day 3 after OSM treatment,the aggregation of tissue-like structure was found from day 7 after inducement.Immunofluorescence results revealed that the tissue-like structure had strong expression of albumin,and PAS results displayed the differentiated APHPs obtained the function of glycogen accumulation.In electron micrographs,cell junction was found and localized along the lateral membrane between cells in cultures of OSM.In addition,microvillis were appeared on one side of plasma membrane.Conclusion The APHPs acquired mature function and structure after OSM treatment in vitro.

关 键 词：肝前体细胞 甲胎蛋白 致瘤素M 细胞分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]