基于SSR标记的谷子遗传多样性研究  

Genetic Diversity Analysis of Foxtail Millet Accessions Revealed by SSR Markers

作　　者：朱学海[1,2] 张艳红[3] 宋燕春[1] 赵治海 刘志斋[1] 石云素[1] 黎裕[1] 王天宇[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]张家口市农业科学院,张家口075000 [3]河北北方学院理学院,张家口075000

出　　处：《植物遗传资源学报》2010年第6期698-702,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(30571168);"863"计划现代农业技术领域课题(2006AA100201);国家科技支撑计划课题(2006BAD13B03)

摘　　要：用21个分布在谷子9条染色体上的SSR标记,对120份来自于核心种质的谷子材料进行遗传多样性研究。21个标记共检查出305个等位变异,各标记检测出的等位变异数在3～26个之间,平均每个位点检测出的等位变异数为14.5个;21个位点的平均多态信息量(PIC)为0.809。基于21个SSR标记的分子鉴定,计算了120份材料间的遗传相似系数,其变化范围为0.8393～0.9672,平均值为0.8906。根据计算的遗传距离,对120份谷子材料进行UPGMA聚类,在遗传相似系数0.8865处这些材料被划分为4个类群,分类结果与这些谷子来源地生态类型总体上表现一致,分别为西北内陆类群、黄土高原内蒙古高原类群、华北平原类群以及华北平原近年育成种类群。120 foxtail millet accessions selected from a core collection were genome-wide fingerprinted by 21 SSR molecular markers covered the 9 chromosomes of foxtail millet for the genetic diversity analysis.The results showed that a total of 305 alleles were captured,ranging from 3 to 26,averaged 14.5 alleles per locus,and the average PIC for all 21 SSRs was 0.809.The genetic similarities between accessions ranged from 0.8393 and 0.9672,with an average of 0.8906,based upon the SSR genotyping.Phylogenetic analysis of all the accessions was performed through the UPGMA method based on the genetic similarities.120 accessions were separated into four clusters with the similarity value of 0.8865.These four clusters based on the SSR genotyping agreed well with the geographical origination of these accessions,which were designated as the names of Northwest Inland Group,Loess Plateau and Inner Mongolia Group,North China Plain Group,and the Synthetic Group of North China Plain.

关 键 词：谷子 SSR 遗传多样性 聚类分析 

分 类 号：S515[农业科学—作物学]