芸薹属作物EST-SNP的发掘与分析  

Detection and Analysis EST-SNP in Brassica

作　　者：李雪姣[1] 张耿[2] 顾爱侠[1] 轩淑欣[1] 王彦华[1] 赵建军[1] 

机构地区：[1]河北农业大学园艺学院,保定071000 [2]浙江大学农业与生物技术学院作物所,杭州310029

出　　处：《植物遗传资源学报》2010年第6期772-776,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(30871713);教育部重点项目(2009);国家科技支撑子课题(2009BADB8B03-1)

摘　　要：芸薹属作物是十字花科中重要的蔬菜和油用作物,经济价值和食用价值比较高。本研究通过生物信息学手段,从3个芸薹属物种EST序列中获得了大量的SNP和Indel资源,对SNP与EST的数量及拼接结果之间的关系、SNP碱基置换的偏好性、Indel碱基数量与发生频率之间的关系进行了分析。分析结果对深入研究芸薹属基因组特点具有一定的参考价值,为进一步通过试验验证预测的SNP位点、开发该作物高通量SNP分子标记奠定了基础。As one of vegetable and oil crops in Cruciferae,genus Brassica has highly economic and edible values.In this research a number of SNPs and indels were obtained from EST sequences of three Brassica species by bioinformatic tools.The relationship between spelling results and the number of SNP and EST,preference of nucleotide replacement,and the relationship between amount and frequency of nucleotides were further analyzed.The results will be useful to gain insight into the characteristics of Brassica genome.The information obtained in the present study is of great importance to test SNP polymorphism experimentally and to develop high throughout SNP molecular markers in Brassica.

关 键 词：芸薹属 序列表达标签(EST) 单核苷酸多态性(SNP) 分子标记 

分 类 号：S33[农业科学—作物遗传育种]