华支睾吸虫胞囊蚴生物学作用的实验观察  

Experimental Observation on Biological Effect of Metacercaria of Clonorchis Sinensis

作　　者：程荣联 张仁平[1] 程廷涛 杨焰[1] 曹勇[1] 喻珊[1] 秦雪松[1] 陈太梅 李家猷 周义芬[1] 

机构地区：[1]重庆市涪陵区疾病预防控制中心,重庆涪陵408000 [2]重庆市涪陵区中医医院

出　　处：《寄生虫病与感染性疾病》2010年第4期173-176,共4页Parasitoses and Infectious Diseases

摘　　要：目的证实华支睾吸虫胞囊蚴是否具有无性生殖生物学作用。方法在非流行区捕获麦穗鱼,经10尾检查证实为阴性后,作为实验用鱼;华支睾吸虫尾蚴由中山大学中山医学院寄生虫学教研室提供。分A、B组作尾蚴定量感染,两次实验相隔30 d。A组将30尾麦穗鱼放在容积为50 cm×30 cm×40 cm的玻璃鱼缸内,11 d内先后放入282条尾蚴感染麦穗鱼;B组用34尾麦穗鱼,分别将每尾鱼单独放在盛有300 m l水的玻杯中,各放入30条尾蚴感染麦穗鱼;感染后的麦穗鱼均在阳台上饲养待查。同时采用直接压片和捣碎水洗沉淀法检查囊蚴。结果 A组实验感染麦穗鱼30尾,丢失1尾,其余29尾感染率为100.00%;共检出囊蚴587个,是感染尾蚴数282条的2.08倍。B组实验感染麦穗鱼34尾,丢失3尾,其余31尾感染率为77.42%;共检出囊蚴1 024个,其中有1尾麦穗鱼检出囊蚴968个,是感染尾蚴数30条的32.27倍。A、B组检出囊蚴数的G值分别为5.84、2.53。感染率与感染度两组间差异有统计学意义(P<0.01、P<0.05)。探讨发生无性生殖的方式,认为有两种即:"囊蚴内繁殖"和"囊蚴外繁殖";无性生殖种原来源于囊蚴内具有生物活性的游离颗粒。结论初步证实华支睾吸虫在第二中间宿主麦穗鱼体内,有第二次无性生殖过程,胞囊蚴具有无性生殖的生物学作用。Objective To demonstrate whether the metacercaria of Clonorchis sinensis has the biological effect of asexual reproduction. Methods Ten negative Topmouth Gudgeon captured in non - endemic area were selected as the experimental fishes ; Clonorchis cercariae were supplied by the Department of Parasitology of Sun Yat - sen University. All the fishes were divided into group A and B for quantitative cercariae infection, and the interval time of the two experiments was 30 days. In Group A, 30 fishes were put into a fishbowl with the volume of 50 cm ×30 cm ×40 cm, and 282 cercariae were put into it to infect the fishes within 11 days. In Group B, 34 fishes were put into the glasses containing 300 ml water separately, and 30 eercariae were put into each glass to infect the fishes. The fishes infected were raised on balcony for experiment. Direct compression tablets and crush washed precipitated methods were used at the same time to check metacercariae. Results Twenty nine fishes were infected with 1 lost in group A, the infection rate was 100.00%. Totally 587 metacercariae were detected, which was 2. 08 times higher than the 282 cercariae. In group B, 3 fishes were lost, and the infection rate among the remaining 31 fishes was 77.42%. Totally 1024 metacercariae were detected, and in 1 fishes, 986 metacercariae were detected, which was 32.27 times higher than others. The G values of the metacercaria numbers in Group A and B were 5.84 and 2.53 respectively. The difference on the infection rate and degree had statistical significance （P 〈 0.01, 0.05 ）. There are two ways to explore asexual reproduction： inner matacercaria reproduction and external matacercaria reproduction; asexual seeds come from the biological active free particles in the matacercariae. Conclusion It is evident that Clonorchis sinensis has a second asexual reproduction process in second intermediate host-Topmouth Gudgeon, metacerearia has biological effect of asexual reproduction.

关 键 词：华支睾吸虫 第二中间宿主 囊蚴 胞囊蚴 无性生殖 

分 类 号：R383.22[医药卫生—医学寄生虫学]