基于石英表面荧光性吖啶橙纳米自组装膜测定土壤中铬(Ⅵ)  

Determination of Chromium(Ⅵ) in Soil by Fluorescent Acridine Orange Nanoparticles Self-assembled Multilayer Films on Quartz Surface

作　　者：胡文英[1] 傅丽君[1] 陈伟建[2] 刘星[3] 

机构地区：[1]莆田学院环境与生命科学系,福建莆田351100 [2]莆田市农业检验监测中心,福建莆田351100 [3]福州大学化学化工学院,福建福州350002

出　　处：《激光生物学报》2010年第5期593-598,共6页Acta Laser Biology Sinica

基　　金：福建省自然科学项目(2010J01045);福建省教育厅科技项目(JK2010048);高校服务海西建设重点项目(2008HX05);莆田市科技计划项目[2009s 02(1)]

摘　　要：研究了在石英表面上吖啶橙纳米金自组装膜的制备及其荧光特性,发现所构建的吖啶橙自组装膜具有强的荧光信号。在pH为7.60的KH_2PO_4-NaOH缓冲溶液中,Cr^(6+)离子能使吖啶橙自组装膜在λ=528 nm处的荧光增强,增强的强度(△F)与Cr^(6+)的浓度在一定浓度范围内呈线性关系,该方法的线性范围为0.00～36.00×10^(-8)μg/mL,检出限(DL)为2.47×10^(-11)μg/mL,将构建的吖啶橙自组装膜测定土壤中的Cr^(6+),回收率为94.91%～96.24%,相对标准偏差(RSD)为0.31%～0.70%。由此可知吖啶橙自组装膜适用于超痕量铬离子的界面荧光测定。实验结果表明该膜具有较好的可逆再生性能,自组装膜法检测限低、灵敏度高、反应速度快、稳定性好。A new fluorescent sensor was proposed by studing the preparation and fluorescence properties of Acridine Orange Self-assembled Monolayer modifided by Gold Nanopartieles on Quartz Surface. The Self-assembled Multilayer Films showed strong fluorescence signal. In the pH 7.60 KH2PO4-NaOH buffer solution, chromium（ Ⅵ ） ions could enhance the fluorescence of Self-assembled Multilayer Films. The enhance strength（△F） showed good linear relationship under a certain concentration range of chromium（ Ⅵ ） ions. The linear range of detection was 0.00 - 36.00 × 10^-8 μg/mL, the detection limit was 2.47 × 10^-11μg/mL. The new fluorescent sensor was used to detect the chromium（Ⅵ） ions in soil of three different areas . The study showed that the recovery ratio with this method was 94.91% -96.24 % , and the relation standard deviaton was from 0.31% to O. 70 %. From which could be seen Aridine Orange Self-assembled membrane was suitable for determinating ultratrace interface fluorescence of chromium（ Ⅵ ） ions. The membrane had good performance on reversible regeneration. The method had wide range, low detection limit, high sensitivity, fast response and good stability.

关 键 词：自组装膜 荧光 吖啶橙 铬(Ⅵ)离子 

分 类 号：O657.3[理学—分析化学]