不同浓度红景天苷对γ射线和质子辐照质粒DNA的影响  

The Protective Effect of Salidroside on DNA Damage Irradiated by Protons and γ Rays

作　　者：孔福全[1] 王潇[1] 隋丽[1] 史晓佩[1] 杨磊[1] 刘建成[1] 倪嵋楠[1] 赵葵[1,2,3] 

机构地区：[1]中国原子能科学研究院,北京102413 [2]河北工业大学理学院,天津300130 [3]北京师范大学射线束技术与材料改性教育部重点实验室,北京100875

出　　处：《激光生物学报》2010年第5期599-602,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(10805078)

摘　　要：利用14.2 MeV的质子和^(60)Co产生的γ射线在200 Gy的剂量下分别对添加了不同浓度红景天苷的pUC19质粒DNA样品进行了辐照,凝胶电泳分析结果表明,红景天苷在两种辐照过程中均对DNA具有一定的保护作用,并且随着浓度的增加保护作用增强。同时还发现质子辐照后DNA样品中出现了线性形态的,而γ射线辐照后则没有出现,再次证实质子对DNA的损伤作用强于γ射线,这应该和质子与物质相互作用复杂以及质子对物质的直接电离作用相关。To measure the protective effect of salidroside on radiation, the plasmid DNA samples in TE Buffers with different concentrations of salidroside were irradiated by 14.2 MeV protons （linear energy transfer = 3.5 keV/Ixm） and γ rays respectively. The images of gel electrophoresis and the DNA forms distribution showed that DNA lesions became lightened with increasing salidroside concentration for both kinds of radiations. At the same time, there were linear DNA forms come forth in DNA samples after irradiated by protons at lower concentrations of salidroside, and that did not appear after irradiated by γ rays. It should be correlative with the direct ionizing of protons.

关 键 词：质子 Γ射线 红景天 质粒DNA 

分 类 号：Q691[生物学—生物物理学]