广西黄瓜绿斑驳花叶病毒(CGMMV)黄瓜分离物的RT-PCR检测及CP基因序列分析  

RT-PCR Detection and CP Gene Sequence Analysis of Cucumber Green Mottle Mosaic Virus Infecting Cucumber in Guangxi,China

作　　者：廖富荣[1] 林石明[1] 秦碧霞[2] 陈青[1] 沈建国[3] 陈涵[1] 黄蓬英[1] 蔡健和[2] 

机构地区：[1]厦门出入境检验检疫局,福建厦门361012 [2]广西农业科学院植保所,广西南宁530007 [3]福建出入境检验检疫局,福建福州350001

出　　处：《激光生物学报》2010年第5期663-668,共6页Acta Laser Biology Sinica

基　　金：国家质检总局科技计划项目(2005IK0061);广西青年科学基金项目(桂科青0640024);福建省自然科学基金项目(2009J01046)

摘　　要：利用黄瓜绿斑驳花叶病毒(Cucumber Green Mottle Mosaic Virus,CGMMV)的特异性引物对来自广西一温室栽培的黄瓜病样进行RT-PCR检测,结果扩增得到了与预期大小相符的目的片段(650 bp)。序列分析表明,该目的片段包含有CGMMV完整的CP基因序列、部分运动蛋白基因(MP)及3'端非编码区(3'-UTR)序列,其中CP基因全长486 bp,与已报道的CP基因序列同源性为91.2%～99.4%。经系统发育分折,明确该GX-CS分离物与日本、法国、印度等分离物属于CGMMV同一类群,并推测该分离物与广西的葫芦(GX-BG)分离物具有相同的起源关系。Cucumber green mottle mosaic virus （CGMMV） was identified on cucumber （Cucumis sativus） grown in a greenhouse in Guangxi, China, by reverse transcription polymerase chain reaction （RT-PCR） with a pair of specific primers of CGMMV. A specific fragment about 650 bp in length, as was expected, was amplified by RT-PCR. Sequences analysis demonstrated that the fragment contained complete coat protein gene CP, partial movement protein gene （MP） and 3＇-UTR of CGMMV. The CP gene of this Guangxi cucumber （GX-CS） isolate had 486 nucleotides in length. The CP gene showed nucleotide homology of 91.2 % to 100.0 % with other isolates or strains. Phylogenetic analyses of these sequences revealed GX-CS isolate was closely related to the Japan, France, India isolates, and maybe have same origin as Guxi GX-BG isolate.

关 键 词：黄瓜绿斑驳花叶病毒 RT—PCR 序列分析 

分 类 号：S432.41[农业科学—植物病理学]