门脉高压大鼠肺微血管周细胞α-SM-actin表达的改变  

Change of α-SM-actin Expression in Pericytes of Pulmonary Microvessel of Portal Hypertension Rat

作　　者：刘中砚[1] 王超[2] 杨镇[2] 

机构地区：[1]同济大学附属同济医院普腹外科,邮政编码上海200065 [2]华中科技大学附属同济医院普外科

出　　处：《微循环学杂志》2010年第4期13-15,F0003,共4页Chinese Journal of Microcirculation

摘　　要：目的:通过对胆汁性肝硬化门脉高压大鼠肺微血管周细胞α-SM-ac-tin表达的测定,探讨门脉高压肺部微血管是否受累,以及周细胞在其中的作用。方法:实验分为三组:模型组、对照组及治疗组(银杏注射液治疗)。应用结扎胆总管复制胆汁性肝硬化门脉高压模型,采用Masson法、免疫组织化学染色、图像分析等技术研究肝硬化门脉高压对大鼠肺微血管周细胞α-SM-actin表达的影响。结果:模型组平均吸光度0.3499±0.089,对照组平均吸光度0.3344±0.0890,治疗组平均吸光度0.2890±0.102,对三组结果进行两两非配对t检验,均具显著性差异(P<0.01)。结论:肝硬化门脉高压可刺激肺微血管周细胞增殖或向平滑肌细胞转化,而银杏注射液能抑制这种改变。这表明门脉高压肺部微血管同样受累,周细胞在病变中可能具有重要的意义。Objective:To study whether pulmonary microvessel is involved and the role that pericytes play in portal hypertension.Method:Portal hypertension model of biliary cirrhosis,Masson staining,immunohistochemistry,image analysis methods were used.Results:It was found that the expression amount of the portal hypertension group(absorbency=0.3499±0.0891),comparison group (absorbency=0.3344±0.0890 ),treated group (absorbency=0.2890±0.1012)were significant different between each two of them.Conclusion:It was suggested that biliary cirrhotic portal hypertension can enhance pericytes' proliferation and transformation to smooth muscles.Ginkgolide injection can inhibit the proliferation and transformation.It also means that microvessel is involved in portal hypertension and pericytes might play an important role.

关 键 词：微血管周细胞 α-SM-actin 门脉高压 血管平滑肌细胞 鼠肺 组织间隙 毛细血管 内皮细胞 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学] R657.34[医药卫生—基础医学]