中药紫萁贯众中紫萁酮的分离及含量测定  

Isolation and Determination of Osmundacetone in Osmundae Rhizoma

作　　者：张东[1] 厉博文[1] 杨岚[1] 付梅红[1] 方婧[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药学杂志》2010年第21期1612-1614,共3页Chinese Pharmaceutical Journal

基　　金：<中国药典>2010年版标准研究(YZ-241)

摘　　要：目的建立中药紫萁贯众的含量测定方法。方法用柱色谱法从紫萁贯众中分离紫萁酮,对紫萁酮HPLC含量测定色谱条件进行优选。结果分离得到紫萁酮化学单体作为含量测定对照品。含量测定采用Kromasil C18（4.6 mm×250 mm,5μm）色谱柱;乙腈-0.1%磷酸水（17∶83）为流动相;检测波长为332 nm;流速为1 mL.min-1。紫萁酮进样量在1.62~324 ng内与峰面积线性关系良好,r=1.000 0,平均加样回收率为99.90%,RSD为1.67%。不同产地药材中含量为0.026 7%~0.051 7%。结论首次建立了中药紫萁贯众中紫萁酮的HPLC含量测定方法,该方法能有效控制紫萁贯众药材的质量。OBJECTIVE To study the quantitative determination method for Osmundae Rhizoma（the rhizome of Osmunda japonica）.METHODS Osmundacetone was isolated from Osmundae Rhizoma with silica gel column chromatography.HPLC was used to determine osmundacetone content in Osmundae Rhizoma and the optimum condition was chosen.RESULTS Osmundacetone was used as reference standard.The Kromasil C18（4.6 mm×250 mm,5 μm） column was used in HPLC,and mobile phase was composed of acetonitrile-0.1% phosphoric acid（17∶83）.The detection wavelength was at 332 nm.The flow rate was 1 mL·min-1.The linear relationship of osmundacetone was in the rang of 1.62-324 ng（r=1.000 0） and the average recovery was 99.90%（RSD=1.67%）.The contents of osmundacetone in the Osmundae Rhizoma from different locations were 0.026 7%-0.051 7%.CONCLUSION The HPLC method for determining osmundacetone in Osmundae Rhizoma is established for the first time.It can be effectively used to controll the quality of Osmundae Rhizoma.

关 键 词：紫萁贯众 紫萁 紫萁酮 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]