固相萃取-高效液相色谱法分析血浆中的13种药物  

Determination of 13 Drugs in Plasma by High Performance Liquid Chromatography with Solid Phae Extraction

作　　者：张吟[1] 陈一农[1] 陈崇宏[2] 

机构地区：[1]福建医科大学附属第二医院药剂科临床药学室,福建泉州362000 [2]福建医科大学药学院药理学系,福州350004

出　　处：《中国药学杂志》2010年第21期1648-1651,共4页Chinese Pharmaceutical Journal

基　　金：福建省卫生厅青年课题(2007-1-27);泉州市科技局课题(2008Z24)

摘　　要：目的建立弱阳离子交换（WCX）固相萃取法提取血浆中13种主要作用于中枢神经系统的药物并用高效液相色谱-二极管阵列检测器（HPLC-DAD）分析。方法血浆经WCX小柱固相萃取后,在Agilent TC-C18色谱柱上,以磷酸盐缓冲液-乙腈为流动相,采用梯度洗脱,流速为1.5 mL.min-1,扫描波长：200~364 nm;检测波长：210 nm。结果 13种药物的萃取回收率72.60%~110.24%,精密度均小于11.55%,检测限0.01~0.10 mg.L-1,线性相关系数在0.998 5以上。结论本法快速、灵敏、重现性好,可用于同时分析测定血浆中的13种药物。OBJECTIVE To establish a SPE-HPLC method for the determination of 13 central nervous system drugs.METHODS Plasma was pretreated by SPE using WCX cartridge.The separation was performed on an Agilent TC-C18 column with mobile phase consisting of acetonitrile-phosphate buffer solution by gradient elution.The flow rate was 1.5 mL·min-1.The detection was set at 210 nm and full spectra were recorded from 200-364 nm.RESULTS The absolute recoveries of 13 drugs were in the range of 72.60%-110.24% with the precisions 11.55%.The limits of detection（LOD）were in the range of 0.01-0.10 mg·L-1.The method showed excellent linearity（linear correlation coefficient ≥0.998 5）.CONCLUSION The method is effective,simple,reliable and could be used for the determination of 13 drugs simultaneously in plasma.

关 键 词：固相萃取 高效液相二极管阵列检测法 弱阳离子交换 血药浓度 

分 类 号：R969.1[医药卫生—药理学]