持续低氧对海马神经元凋亡及蛋白表达影响  

Apoptosis and expressions of c-jun,c-fos in rat hippocampal cells in vitro with continuous hypoxia

作　　者：何悦[1] 董兆君[1] 蔡颖[1] 邓有才[1] 赵吉清[1] 

机构地区：[1]第三军医大学军事预防医学院防化医学教研室,重庆400038

出　　处：《中国公共卫生》2010年第11期1397-1398,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671779)

摘　　要：目的观察持续低氧致体外培养SD乳大鼠海马神经元细胞凋亡及c-jun、c-fos的表达情况。方法海马神经元原代细胞培养,取培养10 d的海马神经元,置于90%N2、5%O2、5%CO2混合气体培养箱中培养2,6,12,24h后,Hoechst 33258染色观察神经元细胞凋亡情况,并用蛋白印迹法检测c-jun、c-fos蛋白表达水平。结果随缺氧时间延长,凋亡细胞所占比例由(23.79±3.43)%上升至(74.36±5.58)%;c-jun、c-fos蛋白在缺氧2 h时表达增高,6 h时达高峰,之后逐渐降低,24 h时仍略高于正常对照。结论持续低氧可引起体外培养海马神经元c-jun、c-fos蛋白表达升高,这可能与神经细胞凋亡以及保护机制有关。Objective To observe the cell apoptosis and the expressions of c-jun,c-fos in neonatal SD rat hippocampal cells with continuous hypoxia in vitro.Methods After ten-days primary culture,the hippocampal cells were exposed to 90% N2,5% O2,5% CO2 for 2,6,12,and 24 hours.The apoptosis of the cells was identified by Hoechst 33258 staining and the expressions of c-jun,c-fos was analyzed with western-blot.Results With the extension period of hypoxia,the number of apoptotic cells was significantly increased from 23.79 ± 3.43% to 74.36 ± 5.58%.Western-blot assay showed the ex-pressions of c-jun,c-fos increased at 2 hr,and reached the highest at 6 hr and then gradually decreased,but were still higher than those of the control at 24 hr.Conclusion Continuous hypoxia can induce the expression of c-jun,c-fos in hippocampal cells in vitro,which might be related to the cell apoptosis and the neuron protective mechanism.

关 键 词：持续低氧 海马神经元 凋亡 C-JUN C-FOS 

分 类 号：Q512[生物学—生物化学]