芩丹颗粒对微波辐射损伤大鼠信号转导影响  

Effect of Qindan particles on signal transduction in rats with microwave radiation damage

作　　者：姜艳霞[1] 徐俊杰[1] 芦晓晶[1] 李妍[1] 王程[1] 罗军[1] 吕士杰 

机构地区：[1]吉林医药学院生化教研室,吉林132013

出　　处：《中国公共卫生》2010年第11期1404-1405,共2页Chinese Journal of Public Health

基　　金：吉林省科技厅白求恩基金(200705407)

摘　　要：目的探讨芩丹颗粒对微波辐射损伤大鼠的防护作用。方法制作微波辐射大鼠模型,检测微波辐射前后体温变化,用芩丹颗粒进行干预,检测各组大鼠血清环化腺苷酸(cAMP)、蛋白激酶A(PKA)、促肾上腺激素(ACTH)、β-内啡肽(β-EP)、热休克蛋白70(Hsp 70)。结果微波辐射后体温明显高于辐射前(P<0.05);微波辐射后1 d,辐射模型组血清中各指标与空白对照组无明显差别(P>0.05);照射后2周,辐射模型组cAMP、β-EP、ACTH、PKA、Hsp 70分别为(23.49±8.96)nmol/L、(12.68±2.21),(86.39±28.88)pg/mL、(4.94±1.21)、(1.73±0.27)ng/mL,明显高于空白对照组(P<0.05),芩丹颗粒组cAMP(10.09±1.03)nmol/L、PKA(2.62±0.55)ng/mL、β-EP(4.61±0.69)pg/mL、Hsp 70(1.63±0.20 ng/mL)明显低于辐射模型组(P<0.05)。结论芩丹颗粒可通过调节cAMP、PKA、β-EP、ACTH含量对微波辐射的损伤起到防护作用。Objective To research protective effect of Qindan particles on microwave radiation damage.Methods The model of microwave radiation in rat was established.The body temperature change of the rats was detected before and after the radiation.After treated with Qindan particles,the serum cyclic adenosine-3＇-5＇ monophosphate（cAMP）,protein ki-nase A（PKA）,adreno-cortic-tropic hormone（ACTH）,β-endophin（β-EP）,and heat shock protein 70（Hsp70） were detec-ted.Results The body temperature of the rats was obviously higher after microwave radiation（P 0.05）.One day after the microwave radiation,cAMP,PKA,ACTH,β-EP and Hsp70 of microwave radiation model group were not obviously differ-ent from those of the control group（P 0.05 for all）.Two weeks after the radiation,cAMP（23.49 ± 8.96 nmol/L）,PKA（4.94 ±1.21 ng/mL）,β-EP（12.68 ±2.21 pg/mL）,ACTH（86.39 ±28.88 pg/mL）,and Hsp70（1.73 ±0.27 ng/mL） of microwave radiation model group were significantly different from those of the control group（P 0.05 for all）.The con-tents of cAMP（10.09 ± 1.03 nmol/L）,PKA（2.62 ± 0.55 ng /mL）,β-EP（4.61 ± 0.69 pg /mL）,and sHsp70（1.63 ± 0.20 ng /mL） of Qindan particles group decreased obviously compared to those of microwave radiation model group（P 0.05 for all）.Conclusion Qindan particles may protect the rats from microwave radiation damage by regulating serum cAMP,PKA,β-EP,and ACTH.

关 键 词：微波辐射 环化腺苷酸 蛋白激酶A 促肾上腺皮质激素 Β-内啡呔 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]