健康人血浆中内源性左卡尼汀及其酰化物的浓度研究  

Study on Plasma Concentrations of L-carnitine and Its Acyl Esters in Healthy Volunteers

作　　者：荆凡波[1,2] 曲海军[1] 朱莉 王春波[2] 曹玉[1] 隋忠国[1] 

机构地区：[1]青岛大学医学院附属医院,青岛市266003 [2]青岛大学医学院,青岛市266071

出　　处：《中国药房》2010年第42期3965-3967,共3页China Pharmacy

基　　金：科技部国家科技支撑计划(2008BAI54B02)

摘　　要：目的:探讨健康中国人食用标准饮食时血浆中内源性左卡尼汀(LC)及其酰化物浓度的变化,对其内源性LC及其酰化物的水平作系统的研究。方法:12名健康受试者均食用标准饮食,分别于禁食10h后,于0、0.5、1.0、1.5、2.0、2.5、3.0、3.5、4.0、5.0、6.0、8.0、12.0、24.0h时取5mL前臂静脉血,分离血浆后,-20℃保存。采用高效液相色谱-荧光法测定血浆中LC、乙酰左卡尼汀(ALC)、丙酰左卡尼汀(PLC)的浓度。结果:食用标准饮食时,LC、ALC、PLC0h的血药浓度分别为(40.25±5.32)、(2.33±0.62)、(0.61±0.22)μmol·L-1;平均血药浓度分别为(40.31±5.44)、(2.34±0.47)、(0.62±0.15)μmol·L-1。各时间点、不同性别受试者之间的血药浓度差异均无统计学意义(P>0.05)。结论:本方法可用作人体内源性LC及其酰化物血浆浓度的检测。OBJECTIVE：To investigate the plasma concentration of L-carnitine（LC）and its acyl esters（ALC,PLC）in the healthy Chinese volunteers given standard diet,and to study the levels of endogenous LC and its acyl esters systematically.METHODS：12 healthy subjects given standard diet were selected.5 mL blood sample of forearm vein was collected at 0,0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,5.0,6.0,8.0,12.0,24.0 h after 10 h of fasting.Plasma was separated and kept at-20 ℃.The concentrations of LC,ALC and PLC in plasma were measured by HPLC-fluorescence method.RESULTS：The plasma concentrations of LC,ALC and PLC at 0 h were（40.25±5.32）μmol · L-1,（2.33±0.62）μmol · L-1 and（0.61±0.22）μmol · L-1 respectively.The mean plasma concentrations of LC,ALC and PLC were（40.31±5.44）μmol · L-1,（2.34±0.47）μmol · L-1 and（0.62±0.15）μmol · L-1,respectively.There was no significant difference in plasma concentration of different sex groups at different time points（P0.05）.CONCLUSION：The method can be used for the plasma concentration determination of LC and its acyl esters.

关 键 词：左卡尼汀 乙酰左卡尼汀 丙酰左卡尼汀 高效液相色谱-荧光法 血浆浓度 

分 类 号：R969.1[医药卫生—药理学] R971[医药卫生—药学]