生物电化学电解池的设计及应用  

Design and applications of bioelectrochemical cell

作　　者：黄河清[1] 林庆梅[2] 

机构地区：[1]厦门大学生物学系,厦门361005 [2]厦门大学环科中心,厦门361005

出　　处：《分析仪器》1999年第2期13-17,共5页Analytical Instrumentation

基　　金：国家自然科学基金!(No.49876027);福建省自然科学基金!(No;96006)

摘　　要：根据生物大分子的生理特性，设计了三种不同的电化学电解池，分别用于研究猪脾铁蛋白、细菌铁蛋白的电化学特性，以及电诱导棕色固氮菌表达新生理特性。说明了电解池的设计特点，给出了电解池示意图。实验结果表明，猪脾铁蛋白及细菌铁蛋白能直接从铂电极上获得还原电子，是非电极惰性蛋白质。棕色固氮菌能在电调控下被诱导成抗氨阻遏的菌株，合成固氮酶。这三种电解他也适合用于分析各种酶、蛋白质及细胞的电化学特性，并具有快速、准确、方便等特点。According to the physiological properties of biological large molecules, three different electrochemical the were designed for studying electrochemical properties of pig spleen ferritin (PSF) and bacterial ferritin, and for electrically inducing azotobacter vinelandii cell (AV all) to express new physiological properties. The diagrammatic sketches of the electrochemical Cells are given and their characteristics described. Experimental results indicate that PSF and bacterial ferritin are able the to obtain reductive elec- trons from platinum electrode directly, showing that they are electrically active on the electrode, and AV cells can be successfully induced into ammonium-resistant strain. The applications show that these cells are.applicable for analyzing enzymes, proteins and living cells and have the advantages of rapidity, convenience and accuracy.

关 键 词：电化学电解池 铁蛋白 棕色固氮菌 蛋白质 设计 

分 类 号：Q503[生物学—生物化学] Q5－33