诱导分化骨髓干细胞(MSCs)构建膀胱的实验研究  

To Construct Tissue-engineered Bladder with Bone Marrow Derived Mesenchymal Stem Cell

作　　者：王忠[1] 李森[2] 

机构地区：[1]广东药学院附属第一医院,广东广州510080 [2]广东省中山市中医院,广东中山528400

出　　处：《临床医学工程》2010年第11期17-18,共2页Clinical Medicine & Engineering

基　　金：中山市科技局资助(20073A187);广东药学院资助(43542046)

摘　　要：目的分离提起大鼠骨髓间充质干细胞(MSCs),体外培养扩增SD大鼠骨髓间充质干细胞(MSCs),通过组织工程技术,构建组织构建膀胱,为进一步临床应用奠定基础。方法将SD大鼠骨髓间充质干细胞进行体外培养扩增后,以生长状态良好的骨髓间充质干细胞接种于制备好的聚乳酸-聚乙醇酸支架上,进行体外联合培养,构建组织工程皮肤。观察细胞生长情况及组织工程构建膀胱。结果体外培养的SD大鼠骨髓间充质干细胞生长良好,传代扩增容易,骨髓间充质干细胞在聚乳酸-聚乙醇酸基质中生长良好,可体外构建组织工程膀胱。结论通过利用体外扩增培养的骨髓间充质干细胞制备的上皮细胞可以体外聚乳酸-聚乙醇酸支架构建组织工程膀胱。Objective To separate and culture bone marrow derived mesenchymal stem cells （MSCs） from bone marrow of SD rat and to construct tissue-engineered bladdex with the cultured cells. Methods MSCs were separated from bone marrow of SD rat and purified by combining density gradient centrifugation with adhering method. The cultured cells in great shape were seeded onto the surface of PLGA, which was cultured absolutely inside liquid medium. In this way, tissue-engineered bladder was expected to be constructed. The cultured cells were observed growing and proliferating well. Results The cultured cells were observed growing and proliferating well in vitro. With the cells cultured in the PLGA, tissue-engineered bladder could be construted in virto. Conclusion Constuction of bladder with BMSCs as well as PLGA in virto is feasible.

关 键 词：骨髓间充质干细胞 聚乳酸-聚乙醇酸 组织工程膀胱 

分 类 号：R318.16[医药卫生—生物医学工程]