兔骨折合并脑外伤模型血清对鼠骨折愈合影响的实验研究  

Experimental Study of Fracture Healing of Rats after Injecting Serum of Rabbits with Fractures and Traumatic Brain Injuries

作　　者：杨运发[1,2] 林博川[1,2] 李远辉[1,2] 张光明[1,2] 徐中和[1,2] 

机构地区：[1]广州市第一人民医院 [2]广州医学院附属广州市第一人民医院骨科,广州510180

出　　处：《临床医学工程》2010年第11期25-27,共3页Clinical Medicine & Engineering

基　　金：广州市医药卫生科技基金项目(2008-YB-016);广东省医学科学技术基金项目(A2009512)

摘　　要：目的在鼠单纯骨折模型中应用兔骨折合并脑外伤模型血清,分析其骨折愈合过程。方法制备兔骨折合并脑外伤模型8只,并制备兔骨折合并脑外伤模型动物不同时段血清(实验血清),分术后1w、2w、3w、4w分别保存,冻干处理;从2只健康兔动脉血制备血清(阴性对照血清),冻干处理;制备鼠单纯股骨干骨折模型72只,骨折行克氏针髓内固定;将单纯股骨干骨折模型鼠随机分成3组,即实验组、阴性对照组和空白对照组,每组24只,据鼠与兔体表面积比计算血清用量,实验组在术后1天(第1周)、8天(第2周)、15天(第3周)、22天(第4周)分别臀肌内注射相应时段实验血清,阴性对照组对应时间肌注阴性对照血清,空白对照组不予任何处理;每组术后1w、2w、3w、4w处死6只鼠,X线摄片比较各组骨折固定术后1w、2w、3w、4w骨折愈合情况。软件分析骨痂形成情况。结果术后2w、3w、4w实验组较对照组骨折处骨痂形成明显加速,骨痂灰度明显致密。结论肌注相应时段兔骨折合并脑外伤模型血清,能明显加速鼠单纯骨折模型的骨折愈合。Objective To analyze fracture healing of the rats after injecting serum of the rabbits with fractures and traumatic brain injuries. Mehtods 8 rabbits with femoral fracture and traumatic brain injury model were prepared, and then the serum of the 8 rabbits （experimental serum） was prepared, lyophilized and saved at 1 w, 2 w, 3 w, 4 w, postoperatively. Negative control serum of 2 healthy rabbits was also prepared, lyophilized and saved. 72 rats with femoral shaft fractures （fixed with Kirschner wire intramedullary） were randomly divided into 3 groups： the ex- perimental group, negative control group and blank control group. The homologous interval experimental serum were intramuscular injected into the rats of the experimental group at 1 d （week 1）, 8 days （week 2）, 15 days （week 3）, 22 days （week 4） after surgery, while negative control serum were intramuscular injected into the rats of the negative control group correspondingly. The serum dose was calculated according to the superficial area rate of the rats and rabbits. The rats of the blank control group had not any management. 6 rats of each group were sacrificed at 1 w, 2 w, 3 w, 4 w postoperatively, and their fracture healing was analyzed by X-ray and callus density analyzed by VeeYooPACS image analysis system. Results The callus formation were more rapid and callus were more dense in experimental group than in the control group at 2 w, 3 w, 4 w, postoperatively. Conclusion The rats will have a rapid fracture healing process after injecting serum ofthe rabbits with fractures and traumatic brain injuries.

关 键 词：骨折 脑外伤 异种动物模型 骨折愈合 实验研究 

分 类 号：R683.42[医药卫生—骨科学] R651.15[医药卫生—外科学]