双色实时荧光PCR方法结合标本混合检测方案快速筛查肠道传染病菌  

Rapid detection of intestinal pathogenes by real time PCR method combined with sample pooling process

作　　者：李义强[1] 杨福荣[1] 孔敏玲[1] 秦小洁[1] 麦洁梅[1] 

机构地区：[1]广州市番禺区疾病预防控制中心,广东广州511400

出　　处：《中国当代医药》2010年第31期78-80,共3页China Modern Medicine

基　　金：广东省广州市番禺区科技计划项目(2009-Z-113-1)

摘　　要：目的:采用双色实时荧光PCR方法结合标本混合检测方案快速筛查检测公共场所从业人员肠道传染病菌,并与经典的培养法比较,探讨该方法的可行性。方法:随机采集5000份从业人员的肛拭子标本,分别采用双色实时荧光PCR方法结合标本混合检测方案和"金标准"培养法同时检测肠道沙门和志贺菌,比较两种方法检测结果之间的差异。结果:双色实时荧光PCR方法结合标本混合检测方案的灵敏度为100%,特异性为99.64%,同培养法相比,具有省时、省力、操作安全、成本接近等优点,两种检测方法的阳性率无显著性差异。结论:提示双色实时荧光PCR方法结合标本混合检测方案可做为从业人员肠道传染病菌快速筛查的技术方法。Objective：The real time PCR method combined with sample pooling process was used to detect the intestinal pathogen in public sanitation related employees,its potential application was evaluated by compared with classical culture method.Methods：5 000 anal swab specimens from public sanitation related employee were randomly collected,the samples were detected simultaneously for Salmonella and Shigella by real time PCR method combined with sample pooling process and ＂gold standard＂ culture method,the sensitivity and specificity of two were also evaluated.Results：The sensitivity and specificity of real time PCR method were 100% and 99.64% respectively,the positive rate showed no significant differences between two methods,however,the real time PCR method was faster,more secure and less laborious consuming compared with culture method.Conclusion：Real time PCR method combined with sample pooling process can be applied on the routine detection of intestinal pathogen in public sanitation related employee.

关 键 词：双色实时荧光PCR 标本混合检测方案 特异度 灵敏度 阳性率 

分 类 号：R446.5[医药卫生—诊断学]