黑果腺肋花楸原生质体分离研究  

Study on Protoplast Isolation of Aronia melanocarpa Elliott

作　　者：岳超君[1] 张志东[1] 吴林[1] 李亚东[1] 杨瑞芹[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118

出　　处：《吉林农业大学学报》2010年第6期639-643,649,共6页Journal of Jilin Agricultural University

基　　金：公益性行业(农业)科研专项(nyhyzx07-028;2006-G25);农业部948"十一五"重大专项(2006-G25);吉林省科技发展计划项目(20080713;20060714;20040112;20075013);长春市科技局项目(长科技合2009153号)

摘　　要：为建立有效的原生质体分离体系,研究了黑果腺肋花楸(Aronia melanocarpa)原生质体酶解分离纯化条件。结果表明:黑果腺肋花楸愈伤组织在20g/L纤维素酶+0.5g/L果胶酶+13%甘露醇+5mmol/LMES酶解液中黑暗静置酶解10h,可获得高产量高活力的原生质体;采用500r/min离心8min分离纯化效果最好;愈伤组织比组培苗叶片分离获得的原生质体产量与活力更高。The establishment of efficient protoplast isolation system is one of the essential conditions to cultivar improvement by using protoplast fusion technique. Studied on conditions of enzymolysis isolation and purification of Aronia melanocarpa were conducted. The results showed that： the optimized combination to obtain protoplast with high yield and activity is 20 g/L Cellulase ＋ 0.5 g/L Pectinase ＋ 13% mannitol ＋ 5 mmol/L MES for 10 h at dark and static condition; centrifugation at 500 r/min for 8 min; from callus of Aronia melanocarpa rather than from leaves.

关 键 词：黑果腺肋花楸 原生质体 分离 纯化 

分 类 号：S663.9[农业科学—果树学]