丹参酮ⅡA对腹膜透析液诱导的大鼠腹膜组织学及氧化应激变化的影响  

The Affects of Tanshinone ⅡA on the Changes of Morphology of Peritoneal Membranes and Oxidative Stress Status in Rat

作　　者：张苗[1] 陶娜娜[1] 蒋春明[1] 孙琤[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肾脏内科,江苏南京210008

出　　处：《药物生物技术》2010年第5期412-416,共5页Pharmaceutical Biotechnology

基　　金：南京市医学科技发展重点项目(ZKX08026)

摘　　要：为了观察丹参酮ⅡA对腹腔注射腹膜透析液(PDS)大鼠腹膜形态以及全身和腹腔局部氧化应激反应的影响,将40只SD雄性大鼠随机分为4组:对照组,每日腹腔注射生理盐水20mL;PDS组,每日腹腔注射4.25%PDS20mL;丹参酮低、高浓度组,每日分别腹腔注射含丹参酮ⅡA浓度为50mg/L、100mg/L的4.25%PDS20mL。于实验第30天,取壁层腹膜行光镜检查,并检测血清和透出液中MDA、SOD、GSH浓度。结果表明HE、Masson染色显示,在PDS干预下,腹膜厚度显著增厚,胶原沉积显著增多,血清及透出液中MDA含量显著升高,SOD水平和GSH水平显著降低。与PDS组比较,用丹参酮的两组腹膜厚度减少,MDA水平显著降低,SOD水平和GSH水平都显著升高。丹参酮IIA具有潜在抑制葡萄糖PDS导致的实验大鼠腹膜纤维化及氧化应激的作用。To investigate the effects of tanshinone ⅡA on the alterations in morphology of peritoneal membranes and the oxidative stress status in a rat model of peritoneal dialysis,fourty male Sprague-Dawley（SD）rats were randomly divided into four groups（n=10 in each group）.Intraperitoneal injection of 0.9% saline,standard lactate-buffered 4.25% glucose-based PDS,and standard lactate-buffered 4.25% glucose-based PDS with various concentrations of tanshinone IIA（50,100 mg/L）was performed for rats in each group once daily respectively for 30 days.Then,histological analyses,including hematoxylin and eosin（HE）staining and Masson staining,were carried out in parietal peritoneum.The samples of effluent and serum were collected to determine the oxidative stress status in local abdomen and the whole body.As compared with control group,chronic high-glucose-based PDS exposure resulted in the increased submesothelial compact zone thickness and accumulation of submesothelial matrix,and significantly high concentration of MDA and low concentration of SOD and GSH were in PDS group.Concomitant PDS and tanshinone IIA exposure drastically reduced the thickness of submesothelial and alleviated the accumulation of submesothelial matrix and increased effluent and serum levels of SOD and GSH as well as decreased level of MDA were detected in tanshinone IIA group as compared with PDS group.Long-term exposure to high glucose-based PDS results in damages in peritoneal membranes and induces the high oxidative stress status in both local and the whole body.Tanshinone IIA may be helpful in ameliorating the deterioration of peritoneal membrane and inhibiting the high oxidative stress status induced by PDS.

关 键 词：丹参酮ⅡA 腹膜纤维化 氧化应激 

分 类 号：R965[医药卫生—药理学]