毛竹种质分子鉴别SRAP、AFLP、ISSR联合分析  

Joint Analysis of SRAP,AFLP,ISSR Molecular Identification for Phyllostachys edulis(Poaceae) Germplasm

作　　者：郭起荣[1,2] 任立宁[1] 牟少华[1] 徐振国[1] 孙立方[1] 王青[1,3] 

机构地区：[1]国际竹藤网络中心,北京100102 [2]江西农业大学江西省竹子种质资源与利用重点实验室,江西南昌330013 [3]北京林业大学,北京100083

出　　处：《江西农业大学学报》2010年第5期982-986,991,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：科技部科技基础条件平台资助(2005DKA21003)

摘　　要：选择10份毛竹(Phyllostachys edulis)种质,突破以往单一分子鉴别的分析方法,采用AFLP、ISSR、SRAP3种标记数据联合进行判别分析,结果显示:联合鉴别分析可以有效提高种质鉴别率,其效果顺序为(AFLP+IS-SR+SRAP)>(AFLP+SRAP)>(ISSR+SRAP)>(AFLP+ISSR);单一鉴别的一致性顺序为SRAP>AFLP>ISSR。探讨了毛竹种质-Q、油毛竹等种质的分子遗传特征。10 accessions of different germplasms of moso bamboo（Phyllostachys edulis cv.Epruinosa,Ph.edulis cv.Huamozhu,Ph.edulis cv.Heterocycla,Ph.edulis cv.Pachyloen,Ph.edulis cv.Tubaeformis,Ph.edulis cv.Nabeshimana,Ph.edulis cv.Gimmet,Ph.edulis cv.Flexuosa and 2 accessions of special moso genetic resources moso -M,moso -Q） and 2 outgroups（Ph.kwangsiensis,Ph.reticulata） are taken for the study：AFLP,ISSR,SRAP,（AFLP ＋ SRAP）,（ISSR ＋ SRAP）,（AFLP ＋ ISSR）,（AFLP ＋ ISSR ＋ SRAP）,single-species and multi-joint identifications method are respectively uesd for the genetic similarity cluster analysis and principal coordinates analysis of genetic distance.Based on the comprehensive analysis of support and resolution,the order of combined identification result is：（AFLP ＋ ISSR ＋ SRAP） （AFLP ＋ SRAP） （ISSR ＋ SRAP） （AFLP ＋ ISSR）,and the result of combined analysis is superior to the effect of a single identification.This study indicates that the genetic similarity coefficient range of the 10 bamboos in the test is 0.599 7-0.869 3,the genetic distance 0.203 8-0.676 4,the sequence homology 97.40%-99.9%,the variation rate 0.0～2.06%.Tag information shows that Ph.edulis cv.Epruinosaan and Ph.edulis cv.Pachyloen are of unique germplasm with special genetic basis.In terms of Ph.kwangsiensis,the results show that not only genetic diversity out sequence analysis on Ph.edulis germplasm,did not show symptoms of overseas groups.

关 键 词：毛竹 种质 AFLP ISSR SRAP 联合鉴别 

分 类 号：Q344.14[生物学—遗传学]