桂花苯丙氨酸解氨酶基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase Gene from Osmanthus fragrans

作　　者：张威威[1] 许锋[1] 张芙蓉[1] 王燕[1] 程水源[2] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]黄冈师范学院生命科学与工程学院,湖北黄州438000

出　　处：《华北农学报》2010年第5期56-60,共5页Acta Agriculturae Boreali-Sinica

基　　金：教育部2005年新世纪优秀人才支持计划(NCET-04-0746);湖北省教育厅高校产学研合作资助项目(CXY2009B009);长江大学博士基金(0010113)

摘　　要：利用一对简并引物,从桂花(Osmanthus fragrans)中克隆得到苯丙氨酸解氨酶(PAL)基因的cDNA片段,命名为OfPAL,GenBank登录号为GU991822。OfPAL长866bp,编码288个氨基酸。通过核苷酸和蛋白质序列多重比较,发现OfPAL与其他植物的PAL基因高度同源。OfPAL编码的蛋白质序列包含与橄榄、烟草、辣椒PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树显示,OfPAL与菊科植物的PAL基因亲缘关系较近。本研究通过克隆OfPAL基因,可为桂花对不良环境的抵御能力以及桂花类黄酮积累方面的研究奠定基础。A phenylalanine ammonia-lyase（PAL） gene cDNA was cloned from Osmanthus fragrans by using a pair of degenerated primers. The PAL cDNA fragment of O. fragrans（designated as OfPAL） was 866 bp length and encoded a 288 amino acid protein. OfPAL was found to have extensive homology with those of other plant PAL genes via multiple alignments. The deamination sites and catalytic active sites in PAL protein of Canarium album,Nicotiana tabacum and Capsicum annuum were also found in OfPAL. Phylogenetic tree analysis revealed that OfPAL had closer relationship with PALs from Asteraceae plants than from other plants. The cloning of OfPAL could be a basis for the study of O. fragrans on its resistant to the environment and the accumulation of flavonoids.

关 键 词：桂花 苯丙氨酸解氨酶 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]