鸡源、猪源大肠杆菌氟苯尼考耐药基因flor的克隆与序列分析  

Study and Analysis on Florfenicol Resistance Gene flor in Escherichia coli from Poultry and Swine

作　　者：郑朝朝[1] 刘聚祥[1] 李辉[1] 刘静[1] 李宏娟 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]秦皇岛市畜牧产业发展协会,河北秦皇岛066300

出　　处：《华北农学报》2010年第5期72-75,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(C2004000329)

摘　　要：为监测大肠杆菌的耐药性,了解不同动物源大肠杆菌氟苯尼考耐药基因flor的差异及其对大肠杆菌耐药性影响,对从保定及其周边地区分离的23株鸡源、14株猪源大肠杆菌进行耐药性测定,并对其氟苯尼考耐药基因flor进行了变异性分析。结果表明,氟苯尼考耐药菌株检出率分别为62%和58%,不同动物源flor基因同源性为99.8%,与GenBank报道的flor基因比较存在3个氨基酸替代,鸡源flor基因在开放阅读框的第439,479,683等位存在点突变,猪源flor基因在开放阅读框的第439,683,1100等位出现点突变。In order to study the resistance and variance of Florfenicol resistance gene（flor） of E. coli from different animals,23 and 14 strains of E. coli isolates respectively from poultry and swine at Baoding region were characterized for drug resistance. And the variance of the flor gene was analyzed with PCR and gene sequencing. The results indicated that 62% isolates from poultry and 58% from swine performanced florfenicol resistance. The homology of the flor gene from different animals was 99. 8% . There were three amino acid substitutions in the flor gene between the sequences coloned and that from GenBank reported. Three point mutations were respectively discovered in the ORF of the gene flor from poutry isolates and swine isolates.

关 键 词：氟苯尼考 耐药性 flor PCR 

分 类 号：Q785[生物学—分子生物学]