口服免疫灭活菌苗对锦鲤幼鱼非特异性免疫功能的影响  

Effect of Oral Immune with Inactivated Bacteria on Non-specific Immunological Function of Juvenile Koi

作　　者：董少杰[1] 梁拥军[2] 孙向军[2] 杨广[1] 任艳[1] 

机构地区：[1]天津农学院水产科学系,天津市水产生态及养殖重点实验室,天津300384 [2]北京市水产科学研究所,北京100068

出　　处：《华北农学报》2010年第5期223-227,共5页Acta Agriculturae Boreali-Sinica

基　　金：天津市自然科学基金项目(07JCYBJC11800);北京市重大项目(D09060500430901)

摘　　要：以锦鲤鱼苗为试验材料,研究了在其发育早期口服免疫迟缓爱德华氏菌和嗜水气单胞菌两种灭活菌苗,对幼鱼非特异性免疫功能的影响。结果表明:自孵化第7天免疫的锦鲤生长至幼鱼后,仅嗜水气单胞菌组头肾指数与对照组差异不显著(P>0.05),其他试验组头肾指数和脾脏指数均高于对照组(P<0.05);嗜水气单胞菌组白细胞吞噬指数PI高于对照组(P<0.01);血清超氧化物歧化酶(SOD)活力均高于对照组(P<0.05)。自孵化第32天免疫的锦鲤生长至幼鱼后,头肾指数与对照组差异均不显著(P>0.05),迟缓爱德华氏菌组脾脏指数高于对照组(P<0.05);白细胞吞噬百分比PP和吞噬指数PI均高于对照组(P<0.01);血清溶菌酶(LZM)活力均高于对照组(P<0.01);血清SOD活力均高于对照组,爱德华氏菌组与对照组差异显著(P<0.05),嗜水气单胞菌组差异极显著(P<0.01)。在锦鲤发育早期口服免疫迟缓爱德华氏菌和嗜水气单胞菌两种灭活细菌苗,可增强其幼鱼的非特异性免疫功能。Effect of oral immunization of Koi fry with inactivated Edwardsiella tarda and Aeromonas hydrophila during early development on non-specific immunological function of juvenile Koi was studied. The result showed that among juvenile Koi fed with inactivated bacteria since the seventh day after hatching,only head kidney weight index in A. hydrophila test group was no remarkable difference with control group（P 0. 05） ,head kidney weight index and spleen weight index in other test groups were obviously higher than control group（P 0. 05） ,phagocytic index of white cell in A. hydrophila test group was highly significantly higher than control group（P 0. 01） ,the activities of SOD in serum of all test groups were obviously stronger than control group（P 0. 05） . Among juvenile Koi fed with bacteria since the thirty-second day after hatching,head kidney weight index in all test groups were not obviously difference with control group（P 0. 05） ,spleen weight index in E. tarda test group was higher than control group （P 0.05） ,phagocytic percent and index of white cell in blood were highly significantly higher than control group （P 0.01） ,the activities of LZM in serum of test groups were highly significantly stronger than control group （P 0.01） ,the activities of SOD in serum of E.tarda test group（P 0.05） and A.hydrophila test group（P 0.01） were stronger than control group. Non-specific immunological function of juvenile Koi could be promoted by taking inactivated E. tarda and A. hydrophila orally in an early period of development.

关 键 词：迟缓爱德华氏菌 嗜水气单胞菌 锦鲤幼鱼 非特异性免疫功能 

分 类 号：S942.5[农业科学—水产养殖]