纳米金刚石药物输运系统的研究  

A study on nanodiamond-based drug delivery system

作　　者：李静[1,2] 诸颖[1] 张小勇[1,2] 李文新[1] 黄庆[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800 [2]中国科学院研究生院,北京100049

出　　处：《核技术》2010年第11期871-875,共5页Nuclear Techniques

摘　　要：利用纳米金刚石对多种分子具有较强吸附能力及团聚时特殊的空间构型,依次将异硫氰酸荧光素(FITC)、10-羟基喜树碱(HCPT)及转铁蛋白(TF),负载于纳米金刚石(NDs)上,形成复合物,使NDs成为具有多种功能的药物载体。使用透射电镜、红外光谱仪、紫外分光光度仪等对NDs及其复合物进行表征及分析,用荧光显微镜及细胞存活率检测方法观察,并检测了复合物的细胞效应。结果表明,HCPT在NDs上3天的吸附量为42.5%;存活率结果显示,复合物组的细胞存活率明显低于单独HCPT组,TF-HCPT-ND复合物组的存活率最低;荧光显微镜下,观察荧光标记的复合物孵育Hela细胞3h后,TF-HCPT-ND组的荧光强度明显高于HCPT-ND组,这与TF-HCPT-ND组的细胞存活率最低的结果相符。本文为构建纳米金刚石的多功能药物靶向输运系统提供了基础。A multifunctional drug delivery system based on nanodiamonds （NDs） has been developed. FITC, HCPT and TF were absorbed on NDs successively to form the multifunetional complex. The NDs and ND complex samples were characterized by TEM, FR-IR and UV-V. The results indicated that this drug delivery system is a high loading system. Efficacy of the drug delivery system on Hela cell was evaluated with MTT assays and fluorescence microscopy. The results show that multiftmetion of the NDs complex include fluorescence, targeting and high efficacy .

关 键 词：纳米金刚石 载体 药物输运 靶向 荧光 

分 类 号：R944.9[医药卫生—药剂学] R318.08[医药卫生—药学]