湖北麻鸡多杀性巴氏杆菌的分离和PCR鉴定  

Isolation and PCR Identification of Avian Pasteurella multocida in Hubei Patridge Chickens

作　　者：邵璐璐[1] 廖永洪[2] 罗玲[2] 罗青平[2] 杨峻[2] 艾地云[2] 温国元[2] 张蓉蓉[2] 王红琳[2] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2010年第11期2663-2664,2668,共3页Hubei Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项资金资助(nycytx-42-G3-04);动物胚胎工程及分子育种湖北省重点实验室基金项目(2010ZD108)

摘　　要：从发病的湖北麻鸡中采集病料,通过细菌分离、生化鉴定,初步判定分离菌株为多杀性巴氏杆菌;应用PCR分别对病料组织和分离菌株进行检测,均能扩增出特异性的条带,PCR产物测序后经NCBIBlast分析,与多杀性巴氏杆菌同源性为99%。经过以上的试验分析,确诊为多杀性巴氏杆菌感染。Clinical amples of infected Hubei patridge chickens were analyzed by a series of experiments,bacteria isolation,physiochemical test,PCR and sequencing analysis,and isolated strains were identified as pasteurella multoida.Specific genes were amplified by PCR from samples and isolated strains,and Blast analysis of amplified genes at NCBI showed 99% homology to pasteurella multoida.Clinical signs and laboratory analysis demonstrated chickens were infected by pasteurella multoida.

关 键 词：多杀性巴氏杆菌 分离 PCR鉴定 

分 类 号：S852.612[农业科学—基础兽医学]