HPLC法测定兔血浆中丹参素、原儿茶酸和原儿茶醛  

Simultaneous determination of danshensu,protocatechuic acid and protocatechuic aldehyde in rabbit plasma by HPLC

作　　者：范菲[1] 房敏峰[1] 王世祥[1] 于洁[1] 余琴兰[2] 郑晓晖[1] 赵桂仿[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西安交通大学中草药现代化研究及工程中心,陕西西安710049

出　　处：《西北大学学报（自然科学版）》2010年第5期843-846,共4页Journal of Northwest University（Natural Science Edition）

基　　金：西北大学研究生交叉学科基金资助项目(07YJC13);陕西省教育厅自然科学基金资助项目(07JK399)

摘　　要：目的建立兔血浆中丹参素、原儿茶酸和原儿茶醛,同时测定高效液相色谱测定方法。方法三氯乙酸沉淀法去除血浆中的蛋白,上清液乙酸乙酯萃取,反相高效液相色谱同时测定丹参素、原儿茶酸和原儿茶醛含量。结果丹参素、原儿茶酸和原儿茶醛检出限分别为0.03,0.08,0.04μg.mL-1,线性范围分别为0.06~40.0μg.mL-1,0.16~100.0μg.mL-1,0.08~50.0μg.mL-1,高、中、低浓度的提取回收率均在82.7%~102.0%之间,日内和日间测定的相对标准偏差在0.56%~4.84%之间。结论测定方法能同时对血浆中丹参素、原儿茶酸和原儿茶醛进行测定,且具有灵敏度高和专一性强的特点,能作为其药物动力学研究方法。Aim To establish an high performance liquid chromatography（HPLC） method for simultaneous determination of danshensu,protocatechuic acid and protocatechuic aldehyde in rabbit plasma.Methods Trichloroacetic acid was used to remove the protein in plasma,and the resulted supernatant was extracted by acetic ether.Then,danshensu,protocatechuic acid and protocatechuic aldehyde were determined by reverse HPLC.Results The detection limits of danshensu,protocatechuic acid and protocatechuic aldehyde were 0.03、0.08 and 0.04 μg·mL-1,respectively,and the linear range were 0.06～40.0 μg·mL-1,0.16～100.0 μg·mL-1 and 0.08～50.0 μg·mL-1,respectively.The recovery of them in low,middle and high concentration was in the range of 82.7%～102.0%,and the precision of inter-and intra-day was among the range of 0.56%～4.84%.Conclusion This method could be used for simultaneous determining danshensu,protocatechuic acid and protocatechuic aldehyde in plasma,with the properties of high sensitivity and specificity.Thus,this method could be used for pharmacokinetic study on danshensu,protocatechuic acid and protocatechuic aldehyde.

关 键 词：丹参素 原儿茶酸 原儿茶醛 HPLC 血浆 

分 类 号：R917[医药卫生—药物分析学]