体外受精妇女血清孕激素对血清和卵泡液白细胞介素-1β水平以及颗粒细胞孕激素受体表达的影响  

Research on relationships between serum progesterone level and IL-1βand follicular fluid and effect on granulose-cell progesterone receptor in IVF women

作　　者：黄琼[1,2] 谭晓珊[1] 谭兵兵[1,2] 张明哲[1] 

机构地区：[1]遵义医学院附属医院生殖中心,563003 [2]贵阳市妇幼保健院妇产科,563003

出　　处：《贵州医药》2010年第10期887-890,共4页Guizhou Medical Journal

摘　　要：目的研究体外受精IVF妇女血清孕激素(P)对血清和卵泡液白细胞介素1β(IL-1β)水平以及颗粒细胞孕激素受体(PR)表达影响。方法根据遵义医学院附属医院生殖中心2007-2008间完成的IVF-ET/ICSI共178周期病历资料,按注射绒毛膜促性腺激素(hCG)日血清P浓度不同分为三组:第1组:P≤2.5 nmol/L;第2组:2.5 nmol/L<P≤5.0 nmol/L;第3组:P>5 nmol/L。同时对其中32例应用酶联免疫吸附双抗体夹心法和免疫组织化学法检测和观察血清和卵泡液IL-1β浓度及卵泡颗粒细胞PR表达的变化,探讨其与血清P水平的关系。结果 hCG注射日血清P浓度和IL-1β浓度及颗粒细胞PR受体灰度值均呈负相关(分别为r=-0.854,P<0.001及r=-0.781,P<0.001)。结论血清P可促进颗粒细胞PR受体的表达,异常水平血清P导致颗粒细胞PR受体表达强度改变。Objective To study the relationships between serum progesterone levels and IL-1βof serum and follicular fluid and effect on granulose-cell progesterone receptor in IVF women.Methods A total of 178 cases which were finished by reproductive center in the Affiliated Hospital of Zunyi Medical College.It was divided into three groups according to inject different hCG doses which were first group（P≤2.5 nmll/L）,second group（2.5 nmll/L P≤5.0 nmll/L）,and third group（P〉5.0 nmll/L）.At same time,32 cases were detected the levels of IL-1βand P in serum and follicular fluid in three groups were compared by ELISA and Immunohistochemistry.Results There were a negative correction between the serum P level and IL-1βlevel（r=-0.854,P〈0.001）.As well as the serum P level and PR gray level of ovarian granulose-cell（r=-0.781,P〈0.001）.Conclusion The results shown that IL-1βmay regulate serum P level.Serum P can accelerate the expression of ovarian granulose-cell PR.

关 键 词：控制性超排卵 体外受精-胚胎移植 白细胞介素-1Β 孕激素受体 

分 类 号：R714.12[医药卫生—妇产科学]