HPLC法同时测定复方制剂中4种钩藤生物碱成分含量  

Simultaneous determination of rhynchophylline,isorhynchophylline,corynoxeine,isocorynoxeine in the traditional Chinese prescriptions by HPLC

作　　者：于晨[1] 杨丽娟[1] 马斌[1] 刘遂库[1] 袁园[2] 袁丹[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]武警四川总队医院,四川乐山614000

出　　处：《沈阳药科大学学报》2010年第11期897-901,共5页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省第二批科学技术计划项目(2005226001);沈阳市科学技术计划项目(1091134-9-00)

摘　　要：目的建立钩藤复方制剂中钩藤碱、异钩藤碱、去氢钩藤碱和异去氢钩藤碱HPLC定量方法。方法 Kromasil C18色谱柱（250 mm×4.6 mm,5μm）,流动相：甲醇-乙腈-醋酸铵缓冲液（pH=5.2）梯度洗脱,流速：1 mL.min-1,检测波长：245 nm,柱温45℃。结果 4种生物碱线性分别为：钩藤碱（rhynchophylline,RIN）：1.76~26.40 mg.L-1,异钩藤碱（isorhynchophylline,IRN）：1.66~24.90 mg.L-1,去氢钩藤碱（corynoxeine,COR）：2.50~37.50 mg.L-1,异去氢钩藤碱（isocorynox-eine,ICO）：1.64~24.60 mg.L-1,r均大于0.999 7,平均回收率分别为98.3%~103.4%,RSD〈2.5%。采用上述HPLC测定了天麻钩藤饮模型制剂和3种市售钩藤复方制剂中4种钩藤生物碱成分的含量。结论本法可作为复方制剂中4种钩藤生物碱成分的含量测定。Objective To quantitatively determine rhynchophylline,isorhynchophylline,corynoxeine and isocorynoxeine of the traditional Chinese prescriptions containing Uncariae Ramulus Cum Uncis using HPLC method.Methods The analysis was carried out in an Kromasil C18（250 mm×4.6 mm,5 μm）.The mobile phase was composed of a gradient elution containing 0.05 mol·L-1 ammonium acetate aqueous and methanol-acetonitrile（50∶50）at the flow rate of 1 mL·min-1.The detection wavelength was set at 245 nm,and the column temperature was at 45 ℃.Results The linear ranges of rhynchophylline,isorhynchophylline,corynoxeine and isocorynoxeine were 1.76-26.40,1.66-24.90,2.50-37.50 and 1.64-24.60 mg·L-1,respectively,with r of 1.000.The recoveries ranged from 98.3% to 103.4%with RSD less than or equal to 2.5%.The contents of four alkaloids of the model preparation of Tianma-gouteng-yin and three commercial preparations containing Uncariae Ramulus Cum Uncis were analyzed using developed HPLC method.Conclusions Because of a good accuracy and a simple procedure,the HPLC method may be practicable for the quantitative analysis of the four rhynchophylline-type alkaloids of traditional Chinese prescriptions.

关 键 词：钩藤生物碱 复方制剂 高效液相色谱法 天麻钩藤饮 定量分析 

分 类 号：R917[医药卫生—药物分析学]