丹酚酸B对鸡胚绒毛尿囊膜血管新生作用的影响  

Effects of salvianolic acid B(sal B)on angiogenesis of the chick embryo chorioallantoic membrane

作　　者：李玉娟[1] 段昌令[1] 李鸿海[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《沈阳药科大学学报》2010年第11期905-909,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金重点资助项目(30830118)

摘　　要：目的探讨丹酚酸B对鸡胚绒毛尿囊膜血管新生的影响。方法将7日龄鸡胚制备鸡胚绒毛尿囊膜(chick embryo chorioallantoic membrane,CAM)模型,随机分为6个组,分别为PBS(phosphatebuffer solution)对照组,丹酚酸B 4个剂量组(150、50、16.67、5.56 mg.L-1)及VEGF(vascular endo-thelial growth factor)组,培养3 d后时采集数据进行评价。结果丹酚酸B 150、50 mg.L-1组血管新生面积明显高于PBS(phosphate buffer solution)对照组(P<0.01,P<0.05)。结论丹酚酸B可明显促进鸡胚绒毛尿囊膜血管新生。Objective To investigate the effect of sal B on angiogenesis of the chick embryo chorioallantoic membrane（CAM）.Methods Forty-eight 7-day-old fertilized eggs were randomly divided into 6 groups,PBS（phosphate buffer solution）group,different concentrations of sal B（150.00,50.00,16.67 and 5.56 mg·L-1）groups and VEGF（vascular endothelial growth factor）group,the chorioallantoic membrane（CAM）models were made,and the carriers with subjects were put on the CAM.After incubation for another 3 days at 37.8 ℃ at constant humidity,their effects were evaluated.Results The angiogenesis area in sal B 150,50 mg·L-1 groups was obviously increased compared with PBS group（P0.01,P0.05）.Conclusions The result shows that sal B can obviously stimulate angiogenesis of the chick embryo CAM.

关 键 词：丹酚酸B 血管新生 鸡胚绒毛尿囊膜模型 

分 类 号：R284.1[医药卫生—中药学]