应用细菌磁颗粒RT-PCR检测3种植物病毒  

The application of bacterial magnetic particales RT-PCR for detecting three kinds plant viruses

作　　者：孙宁[1,2] 张一折[1] 陈继峰[1] 邓丛良[2] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]北京出入境检验检疫局

出　　处：《植物检疫》2010年第6期16-19,共4页Plant Quarantine

基　　金：郑州市科技项目(0910SGYG23257-3)

摘　　要：根据细菌磁颗粒对植物病毒颗粒的非特异性吸附作用,建立了一种新的提取植物病毒RNA的方法。利用细菌磁颗粒收集黄瓜绿斑驳花叶病毒、黄瓜花叶病毒和百合无症病毒等3种病毒颗粒,富集分离后,通过高温使其裂解释放出病毒RNA,进行RT-PCR检测。与利用Trizol试剂提取这3种植物病毒的RNA相比,具有操作简单、无毒性,能够显著缩短提取时间。对黄瓜绿斑驳花叶病毒的检测灵敏度验证表明:BMPs-RT-PCR与Trizol-RT-PCR的检测灵敏度相同,均为105倍的植物叶片组织稀释液。A novel method used to isolate RNA of the plant virus was established,which was based on the non-specific absorption between the bacterial magnetic particles（BMPs）and the plant viruses.The viriones of Cucumber green mottle mosaic viruses（CGMMV）,Cucumber mosaic virus（CMV）,and Lily symptomless virus（LSV）were enriched by bacterial magnetic particles,respectively,and RNAs were released by a applied high temperature.RT-PCR method was used for detection CGMMV,CMV,and LSV.Compared to the extraction method of RNA by utilizing the organic reagents（Trizol reagent）,the BMPs-based method for RNA collection has the advantages of simplicity,nontoxicity and time saving.The sensitivity comparison of the CGMMV detection indicates that BMPs-based RT-PCR and Trizol-based RT-PCR had the same sensitivity of the 105 dilution times of the plant leaf materials.

关 键 词：细菌磁颗粒(细菌磁小体) RT-PCR 黄瓜绿斑驳花叶病毒 黄瓜花叶病毒 百合无症病毒 检测 

分 类 号：S432.41[农业科学—植物病理学] S41-30[农业科学—农业昆虫与害虫防治]