液相芯片技术在植物病毒检疫中的应用展望  

The application and prospect of liquichip in plant virus quarantine

作　　者：代欢欢[1] 陈舜胜[1] 杨翠云[2] 戚龙君[2] 于翠[2] 

机构地区：[1]上海海洋大学,上海201306 [2]上海出入境检验检疫局

出　　处：《植物检疫》2010年第6期44-48,共5页Plant Quarantine

基　　金：国家质检总局科技项目(20091K252)

摘　　要：液相芯片是一个在悬浮液态体系中进行的生物分子间反应,并以100种不同荧光编码的微球作为探针载体的一类新型生物芯片技术。具有高通量、高灵敏度、高准确度、高精密度、宽的线性范围及操作简便等众多优势,可用作蛋白质和核酸等生物分子的高通量检测平台。本文主要介绍了液相芯片的检测原理、优势,并对该技术的应用及在植物检疫中的展望作一简要介绍。Liquichip is a new type of biochip with 100 different kinds of fluorescent beads as carriers of different probes and the reaction of biomolecules is finished in suspenseful liquid system.Featuring many remarkable advantages such as the high-throughput,the high-sensitive,the high-accuracy,the high-precision,the great dynamic range and the easy operation.It provides an open platform for assays of protein,nucleic acid and biomolecular interaction analysis.Here we provided brief introduction of its principle and advantages,and reviewed applications of this new array technology and the prospects in plant virus quarantine.

关 键 词：液相芯片 检测原理 优势 应用 

分 类 号：S41[农业科学—植物保护]