诱导痰液与哮喘患儿气道炎症的关系探讨  

Relationship between induced sputum and airway inflammation in children with asthma

作　　者：盛军 邵洁[1,2] 傅曼芬 李云珠[1,2] 

机构地区：[1]上海嘉定区南翔医院 [2]上海第二医科大学附属瑞金医院

出　　处：《中国实用儿科杂志》1999年第3期163-165,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的探讨简便有效的诱导痰液及细胞分析方法，了解儿童哮喘发作期气道炎症的特点。方法运用高渗盐水雾化吸入诱导痰液的方法，收集３７例哮喘发作期患儿、４８例正常儿童（对照组）痰液。一部分痰液经二硫苏糖醇（ＤＴＴ）液化后，在血球计数板上行细胞总计数；另一部分痰液涂片经瑞氏、甲苯胺蓝染色行细胞分类计数。结果哮喘组８４％取痰成功，诱导过程中呼气峰流速（ＰＥＦ）未见下降（Ｐ＞００５）。痰液细胞总计数哮喘组为（１１２±８９）×１０９／Ｌ，对照组为（７１±６２）×１０９／Ｌ（Ｐ＜００５）。嗜酸性粒细胞、肥大细胞在哮喘发作期气道中明显升高（Ｐ＜００１）。结论诱导痰液分析、评判哮喘患儿气道炎症是一种安全、可靠的新研究方法；嗜酸性细胞、肥大细胞为哮喘发作的主要效应细胞。Objective To explore a simple and effective method of induced sputum and cellular analysis,and to investigate airway inflammation features in children with acute asthma.Methods A method to induce sputum with inhaled hypertonic saline was used in 37 acute asthmatic childhood patients and 48 normal subjects(control group) for collecting the sputum.A portion of the sputum treated with dithiothreitol(DTT) was performed to count the total cell in the hemocytomer and the other did differential cell counts in the smear with Wright's stain and toluidine blue stain.Results In the asthmatic group,84% were successful in collecting the sputum and PEF value did not go down( P >0 05).The total cell counts were(11 2±8 9)×10 9/L in sputum from asthmatic subjects and (7 1±6 2)×10 9/L from the control subjects( P <0 05).The eosinophils and the mast cells of the asthmatic group were significantly higher than that of the control group( P <0 01) in the differential cell counts.Conclusion The analysis of the induced sputum and investigation of the airway inflammation was a safe and reliable method;eosinophils and mast cells were mainly effective cells in inflammatory response in asthma.

关 键 词：儿童 哮喘 气道炎症 痰诱导 痰液分析 

分 类 号：R725.622.5[医药卫生—儿科]