健脾理气方联合rIL-2对荷瘤小鼠化疗免疫抑制的影响  

Effect of Jianpi Liqi Priscription Combined with rIL2 on Immune Supression Caused by Chemotherapy

作　　者：游捷[1,2,3] 宋明志[1,2,3] 黄辉[1,2,3] 于尔辛[1,2,3] 黄雯霞 林云璐[1,2,3] 

机构地区：[1]上海医科大学肿瘤医院中西医结合研究室 [2]基础医学院免疫学教研室 [3]上海友谊医院内科

出　　处：《上海医科大学学报》1999年第2期113-115,共3页Journal of Fudan University(Medical Science)

摘　　要：目的研究联合应用中药健脾理气方和重组白细胞介素－２（ｒＩＬ－２）对肿瘤化学治疗免疫抑制的影响。方法用环磷酰胺（ＣＴＸ）给Ｈ２２荷瘤小鼠化疗，造成免疫抑制，应用中药健脾理气方和ＩＬ－２联合治疗后，用３Ｈ－ＴｄＲ释放法检测荷瘤小鼠的ＮＫ、ＬＡＫ细胞活性，３Ｈ－ＴｄＲ掺入法检测脾细胞增殖活性，ＦＡＣＳ法检测ＩＬ－２受体，Ｌ３Ｔ４，ＬＹ２阳性细胞数。结果采用大剂量ＣＴＸ后，荷瘤鼠脾重、脾细胞数、脾细胞增殖及杀伤能力均明显降低。Ｔ细胞亚群中ＴＨ比例下降明显，ＩＬ－２受体表达能力也受损，而加用健脾理气方或ｒＩＬ－２后，上述各项指标均有一定改善，其中中药组ＴＨ比例升高较明显；两者合用，则各项免疫指标的恢复更加显著。结论中药及生物反应调节剂联合应用有可能会更有效地减轻化疗的免疫抑制作用。Purpose To observe the effect of traditional Chinese prescription of Jianpi Liqi combined with rIL2 on immune suppression caused by chemotherapy. Methods H 22 tumor bearing mice were treated with high dose of CTX to induce immune supression.The level of splenocyte proliferation and cytotoxicity were measured.FACS Analysis was used to detect T cell subsets ratio and IL2 and receptor expression. Results Immune functions were seriously suppressed by high dose of CTX.RIL2 and Jianpi Liqi herbs could improve all these functions.Better results were obtained from the CTX+rIL2+herbs group. Conclusions The combination of herbs and BRM(Biological response modifier) may be a useful model for improving the immune injuries after chemotherapy.

关 键 词：肿瘤 健脾理气方 免疫抑制 荷瘤小鼠 RIL-2 

分 类 号：R273.053[医药卫生—中西医结合] R285.5[医药卫生—中医肿瘤科]