电离辐射对Jurkat细胞γ-H2AX蛋白表达的影响  

Effects of ionizing radiation on expression of γ-H2AX in Jurkat cell lines

作　　者：倪冠英[1,2] 何淑梅[3] 董娟聪[1] 金顺子[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]吉林省肿瘤医院核医学科,吉林长春130012 [3]吉林大学第一医院干部病房,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第5期895-899,共5页Journal of Jilin University：Medicine Edition

基　　金：卫生部公益性行业科研专项基金资助课题(200802018)

摘　　要：目的:探讨电离辐射诱导Jurkat细胞γ-H2AX蛋白表达的影响。方法:采用流式细胞术(FCM)分别检测2.0GyX线照射后不同时间点(0、0.5、1.0、4.0、8.0、16.0及24.0h)和0.5、1.0、2.0、4.0及6.0Gy不同剂量X线照射后Jurkat细胞γ-H2AX表达变化,并于量效实验同时进行Jurkat细胞凋亡率检测。结果:与假照组比较,2.0GyX线照射后Jurkat细胞γ-H2AX表达水平于1.0h内达最大值,1.0h后开始呈时间依赖性下降,16.0h仍高于假照水平(P<0.01),24.0h降至与对照无明显差异;不同剂量X线照射后1.0h,Jurkat细胞γ-H2AX的表达水平在0.5Gy以内没有明显变化,0.5Gy以后呈剂量依赖性升高,4.0Gy时达最高水平(P<0.01),6.0Gy时表达略下降;不同剂量X线照射后8.0h,γ-H2AX表达在0～4.0Gy范围内随剂量增大而逐渐升高,4.0Gy时达到最高水平(P<0.01),6.0Gy时表达有所下降。结论:X射线能诱导Jurkat细胞γ-H2AX表达增高,在一定范围内存在时间和剂量依赖关系。Objective To investigate the effects of ionizing radiation on the expression of γ-H2AX in Jurkat cell lines.Methods The changes of the expressions of γ-H2AX in Jurkat cell lines after X-irradiation with different doses（0.5,1.0,2.0,4.0,and 6.0Gy）and after irradiation with 2.0Gy X-rays for different time （0,0.5,1.0,4.0,8.0,16.0,and 24h）were analyzed by flow cytometry（FCM）,and the apoptotic rate of Jurkat cell lines was detected during dose-effect experiment.Results In time-effect experiment,the results showed that the expression ofγ-H2AX reached to the peak within 1h,followed by a time-dependent decrease,kept expressing at 16.0h,and had no significant difference with control at 24.0h.In dose-effect experiment,it was demonstrated that the expressions of γ-H2AX showed a dose-dependent increase in the range of 0.5-4.0Gy at 1.0hafter exposed to X-rays,and dropped at the does of 6.0Gy;at 8.0hafter exposure to those doses above,the express levels of γ-H2AX enhanced linearly with the dose increased in the range of 0-4.0Gy,showing a clear dose-dependent manner（P〈0.01）.Conclusion The γ-H2AX expression could be increased by X-irradiation in time-dependent and dose-dependent manners.

关 键 词：电离辐射 蛋白表达 Γ-H2AX JURKAT细胞 

分 类 号：R811.5[医药卫生—放射医学]