厄贝沙坦对肾性高血压大鼠肾脏腺苷三磷酸酶活性和血管紧张素Ⅱ的影响  

Effects of irbesartan on ATPase activity and AngⅡ in kidney of renal hypertensive rats

作　　者：吴泽兵[1,2] 商黔惠[1] 姜黔峰[1] 吴芹[3] 袁萍[1] 

机构地区：[1]遵义医学院临床医学研究所遵义医学院附属医院心血管内科,563003 [2]安徽省合肥市第一人民医院干部病房 [3]遵义医学院药理教研室

出　　处：《中华老年心脑血管病杂志》2010年第11期1020-1023,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：贵州省优秀科技教育人才省长专项基金(2005239);贵州省科学技术基金重点项目(20023013)

摘　　要：目的探讨厄贝沙坦对肾性高血压大鼠(RHR)肾脏腺苷三磷酸酶活性及血管紧张素Ⅱ(AngⅡ)的影响。方法采用两肾一夹制造RHR模型18只随机分为模型组、厄贝沙坦组、停药组,每组6只,未造模的6只大鼠为假手术组,用药8周后,用酶学比色法检测大鼠左、右肾皮质Na^+-K^+-ATP酶、Ca^(2+)-ATP酶活性,放射免疫技术测AngⅡ水平。结果与假手术组比较,模型组、厄贝沙坦组和停药组大鼠左右肾皮质Na^+-K^+-ATP酶活性显著降低,血浆AngⅡ显著升高,模型组和停药组Ca^(2+)-ATP酶活性显著降低;与模型组比较,厄贝沙坦组Ca^(2+)-ATP酶活性显著升高,厄贝沙坦组和停药组血浆AngⅡ显著升高;与厄贝沙坦组比较,停药组Ca^(2+)-ATP酶活性显著降低,右肾皮质AngⅡ显著升高。肾脏Ca^(2+)-ATP酶与局部AngⅡ呈显著负相关。结论厄贝沙坦影响RHR肾脏Ca^(2+)-ATP酶活性及AngⅡ水平,停药后AngⅡ及Ca^(2+)-ATP酶活性变化,可能是高血压反跳的机制之一。Objective To explore the effects of irbesartan on activities of Na^＋-K^＋-ATPase,Ca^2＋- ATPase and level of Ang Ⅱ in kidney of RHR. Methods Renovascular hypertension was induced by two kidney-one clip method. Eighteen RHR were randomly divided into RHR group（Mod）, irbesartan group（Irb） and discontinuation of medication group（Dis）. Other 6 SD rats served as sham operation group（Sha）. ATPase activity and Ang Ⅱ content in renal cortexes of RHR were determined by spectrophotometry and radioimmunoassay respectively after 8 weeks of irbesartan treatment. Results Compared with Sha,the activities of Na^＋-K^＋- ATPase of bilateral renal corte- xes were significantly lower, plasma Ang Ⅱ level was significantly higher in Mod, Irb and Dis, the activity of Ca^2＋-ATpase was significantly lower in Mod and Dis. Compared with Mod, the activity of Ca^2＋-ATPase significantly increased in Irb,plasma Ang Ⅱ significantly higher in Irb and Dis. Compared with Irb, the activity of Ca^2＋-ATPase decreased and Ang Ⅱ level significantly increased in Dis. There was a significant negative correlation between Ang II and the activity of Ca^2＋- ATPase in kidney. Conclusions Irbesartan influences the activity of Ca^2＋-ATPase and level of Ang Ⅱ in kidney of RI-IR. The Ang Ⅱ and activity of Ca^2＋-ATPase are in disorder after discontinuation of medication, which might be one of the mechanisms of rebound of hypertension.

关 键 词：高血压 肾性 联苯化合物 四唑类 腺苷三磷酸酶 血管紧张素Ⅱ 抗高血压药 厄贝沙坦 

分 类 号：R544.1[医药卫生—心血管疾病]