高蛋白膳食对小鼠胰腺功能的氧化损伤  

The Oxidative Damage of High Protein Diet on Pancreas Function in Mice

作　　者：谷春梅[1,2] 施用晖[2] 乐国伟[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]江南大学食品科学与技术国家重点实验室,江南大学食品学院食品营养与安全研究所,无锡214122

出　　处：《中国畜牧兽医》2010年第11期32-35,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30571347)

摘　　要：为探讨高蛋白膳食对小鼠胰腺功能的影响,试验选用30只C57BL/6J雄性小鼠,按体重随机分成对照组、高蛋白组和抗氧化剂组3组。试验2周后处死小鼠,取胰腺组织进行检测。结果表明,与对照组相比,高蛋白组MDA显著升高(P<0.05),SOD、GSH-Px活性和T-AOC显著下降(P<0.05),脏器中胰腺重影响较大,表现为显著增加,蛋白质、DNA和RNA浓度显著提高,消化酶活性显著降低,生长抑素和胰岛素水平显著提高(P<0.05)。添加抗氧化剂—半胱胺后,自由基水平被降低,抗氧化能力被提高,胰腺功能也有所改善。因此,高蛋白膳食可通过破坏胰腺氧化和抗氧化平衡状态,增加自由基对胰腺内外分泌功能的氧化应激损伤。To estimate the oxidative damage of high protein diet on of pancreas function in mice,thirty male C57BL/6Jmice were randomly divided into three groups：control group,high protein group and cysteamine group.Each group was fed for 2 weeks,and then pancreas samples were collected to examine pancreas function.The results showed that ingestion of high protein diet markedly increased contents of MDA and decreased T-AOC and activities of SOD and GSH-Px（P〈0.05）.Pancreas weight and concentration of protein,DNA and RNA were significantly increased.Digestive enzyme activities were significantly decreased and levels of somatostatin and insulin were enhanced（P〈0.05）.Supplemented cysteamine decreased free radical levels and increased antioxidative capability,and improved pancreas function.These data demonstrated that high protein diet can increase oxidative damage of free radical on pancreas function through destroying balance of oxidant and antioxidant.

关 键 词：高蛋白膳食 胰腺 氧化损伤 抗氧化剂 小鼠 

分 类 号：S852.31[农业科学—基础兽医学]