绵羊DECR1基因exon 5部分序列多态性分析  

Polymorphism Analysis of Exon5 of 2,4-dienoyl-CoA Reductase1 Gene in Sheep

作　　者：刘冬 刘桂林[2] 

机构地区：[1]山西农业大学文理学院,太谷030801 [2]山西农业大学动物科技学院,太谷030801

出　　处：《中国畜牧兽医》2010年第11期132-135,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(2007031056021043)

摘　　要：试验根据GenBank登录的牛2,4-双烯-CoA还原酶1(2,4-dienoyl-CoA reductase1,DECR1)基因序列(NP_001068891)设计引物,应用PCR技术对德国美利奴绵羊、杜泊绵羊、特克塞尔绵羊DECR1基因的exon 5部分序列进行克隆测序;利用PCR-SSCP技术检测了3个绵羊群体exon 5单链构象多态性(SNP),所获序列与GenBank中其他物种exon 5序列进行了同源性比较,并构建了亲缘关系聚类分析图。结果表明,绵羊DECR1基因exon 5长为137bp,3个绵羊群体中exon 5均不存在SNPs,各种动物之间DECR1基因exon 5的同源性较高,在81.02%(鸡)～97.08%(牛)之间,其中绵羊和牛同源性最高,达到97.08%;与鸡的同源性最低,为81.02%;聚类分析结果显示,绵羊首先与牛聚为一类,再分别与兔子、狗聚为一类,最后分别与人、猪聚为一类,绵羊与牛的亲缘关系最近,与鸡的亲缘关系最远,与传统分类相一致,说明DECR1基因exon 5在动物进化过程中高度保守。According to exon 5 sequences published by GenBank of 2,4-dienoyl-CoA reductase 1（DECR1）gene of bovine（NP_001068891）,one pair primer was designed to amplifiy exon 5 of DECR1 gene by using PCR technique for three sheep populations including of German MuttanMerino sheep,Dorper sheep and Texel sheep,the single nucleotide polymorphism of three sheep populations were detected by PCR-SSCP,and compared and analyzed with other species of chicken,rat,sheep,pig,human,bovine,rabbit,dog from GenBank.The results showed that the size of DECR1gene exon 5 was 137 bp,SNPs was not found in exon 5 of DECR1 gene by PCR-SSCP.By means of sequence homology aligment between sequence of exon 5 of sheep DECR1gene and others species,the results showed that homology of different species DECR1gene was 81.02%to 97.08%,the highest similarity was 97.08% between sheep and bovine.The molecular phylogenetic tree constructed by DECR1 gene exon 5sequences showed that sheep clustered with bovine firstly,and secondly with rabbit and dog,and then human and pig.This results indicated that sheep had the most nearest relative relationship with bovine,but the far-thest with chicken.It was consistent with the orthodox taxonomic results,and DECR1 gene was very conservative in the progress of animal evolu-tion.

关 键 词：绵羊 DECR1基因 EXON 5 序列 SNP 

分 类 号：Q753[生物学—分子生物学]