重组人粒细胞集落刺激因子对健康供者脾脏大小的影响及其相关因素研究  

Study on effects of recombinant human granulocyte colony-stimulating factor on spleen size of healthy donors and related factors

作　　者：宋伟[1] 徐娟[1] 赵玥[1] 王萍[2] 金辉 孙雪静[1] 苏力[1] 李涛 冀冰心[1] 

机构地区：[1]首都医科大学宣武医院血液科,北京100053 [2]首都医科大学宣武医院超声波检查科,北京100053 [3]中国造血干细胞捐献者资料库北京管理中心,北京100088

出　　处：《药物不良反应杂志》2010年第5期305-308,共4页Adverse Drug Reactions Journal

基　　金：北京市财政课题调研;规划类项目(PXM2006-077203-009449)

摘　　要：目的:观察重组人粒细胞集落刺激因子(rhG-CSF)对健康造血干细胞捐献者脾脏大小的影响及其相关因素。方法:28例健康供者皮下注射rhG-CSF5μg/(kg.d),连续5d动员造血干细胞。在注射前和最后一次注射后第1、7及30天对健康供者进行脾脏超声检查,测量脾脏大小(长径×厚度);同时计数外周血中性粒细胞数量并检测细胞表面CD11b表达的相对平均荧光强度(MFI)。结果:供者脾脏的大小在注射前为(40.3±3.4)cm2,最后一次注射后第1、7和30天分别为(48.2±6.1)、(47.8±6.6)和(39.2±5.0)cm2,最后一次注射后第1、7天与注射前相比差异均有统计学意义(均P<0.013);供者外周血中性粒细胞数量在注射前为(3.4±1.0)×109/L,最后一次注射后第1和7天分别为(29.3±6.9)×109/L和(3.2±0.7)×109/L,最后一次注射后第1天与注射前相比差异有统计学意义(P<0.017);中性粒细胞表面CD11b表达的相对MFI在注射前为(57.1±43.5),最后一次注射后第1和7天分别为(96.8±51.3)和(66.9±30.4),最后一次注射后第1天与注射前相比差异有统计学意义(P<0.017)。线性回归分析结果显示,最后一次注射后第1天脾脏的增大与外周血中性粒细胞数量及细胞表面CD11b的表达均具有相关性(r=0.603,P=0.000;r=0.436,P=0.023)。结论:rhG-CSF可使健康供者脾脏在短期内明显增大,这与中性粒细胞数量以及细胞表面CD11b的表达相关。Objective：To investigate the effects of recombinant human granulocyte colony-stimulating factor（rhG-CSF）on the spleen size of healthy donors for hematopoietic stem cells and the related factors.Methods：Twenty-eight healthy donors were injected with rhG-CSF 5 μg/（kg·d）subcutaneously for 5 days in order to mobilize hematopoietic stem cell release from bone marrow to peripheral blood.The spleen size（length×thickness）was measured by ultrasound before injection of rhG-CSF and on days 1,7,and 30 after the last injection.At the same time,the number of neutrophils in the peripheral blood was counted and mean fluorescence intension（MFI）of CD11b was determined.Results：The spleen size was（40.3±3.4）cm2 before injection,（48.2±6.1）,（47.8±6.6）,and（39.2±5.0）cm2 on days 1,7,and 30 after the last injection,respectively.The differences in the spleen size were statistically significant between on days 1 and 7 after the last injection and before injection（all P0.013）.The number of neutrophils in peripheral blood was（3.4±1.0）×109/L before injection,（29.3±6.9）×109/L and（3.2±0.7）×109/L on days 1 and 7 after the last injection,respectively.The difference in the number of neutrophils was statistically significant between on day 1 after the last injection and before injection（P0.017）.The relative values of MFI of CD11b expression was 57.1±43.5 before injection,96.8±51.3 and 66.9±30.4 on days 1 and 7 after the last injection,respectively.The difference in the relative values of MFI of CD11b expression was statistically significant between on day 1 after the last injection and before injection（P0.017）.Linear regression analysis showed that spleen enlargement on day 1 after the last injection was related to the number of neutrophils and the expression of CD11b（r=0.603,P=0.000;r=0.436,P=0.023,respectively）.Conclusion：The spleen of healthy donors can be enlarged in a short period of time after rhG-CSF injection,which is related to the number of neutrophils in pe

关 键 词：重组人粒细胞集落刺激因子 健康供者 脾脏 中性粒细胞 CD11B 

分 类 号：R96[医药卫生—药理学]