HPLC测定广西不同产地千里香中九里香酮和5′-九里香酮的含量  

Determination of Murrayone and 5′-Murrayone in Murraya paniculata from Different Habitats in Guangxi by HPLC

作　　者：姜平川[1] 李嘉[1] 黄建猷[1] 陈锋[1] 李红[1] 张赟赟[1] 

机构地区：[1]广西中医药研究院,南宁530022

出　　处：《中国实验方剂学杂志》2010年第16期36-38,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科技厅科技攻关项目(桂科攻0815005-1-22)

摘　　要：目的:建立千里香中九里香酮和5′-九里香酮的HPLC含量测定方法,并检测广西不同产地千里香中九里香酮和5′-九里香酮的含量。方法:采用高效液相色谱分析,用Global Chromatography SP-120-5-ODS-AP C18色谱柱(4.6 mm×150 mm,5μm);以甲醇-水(60∶40)为流动相;检测波长332 nm。结果:九里香酮和5′-九里香酮分别在0.589 6～5.896μg和0.564 4～5.64μg呈良好的线性关系,r=0.999 9;平均回收率分别为100.2%(RSD 1.39%)和100.3%(RSD 2.01%)。结论:广西不同产地千里香中九里香酮和5′-九里香酮的含量存在较大差异,以龙州产含量最高。Objective： To determine murrayone and 5′-murrayone in Murraya paniculata from different habitats in Guangxi.Method：A Global Chromatography SP-120-5-ODS-AP C18 Column（4.6 mm × 150 mm,5 μm）was used with methanol-water（60∶40） as the mobile phase and UV detection was at 332nm.Result：The calibration curves were linear in the range of 0.589 6 ～ 5.896 μg for murrayone and 0.564 4～5.64 μg for 5′-murrayone,and the both correlation coefficients（n = 5） were 0.999 9;the average recovery（n = 6） for murrayone and 5′-murrayone were 100.2%（RSD 1.39%） and 100.3%（RSD 2.01%） respectively.Conclusion：There were large differences in the contents of murrayone and 5′-murrayone among murraya paniculata with different habitats in Guangxi.The highest content was found in Longzhou.

关 键 词：千里香 九里香酮 5′-九里香酮 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]