紫外分光光度法测定牛黄至宝丸中胆红素的含量  

Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry

作　　者：刘力丰[1] 

机构地区：[1]长春中医药大学附属医院,长春130021

出　　处：《中国实验方剂学杂志》2010年第16期62-63,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立牛黄至宝丸中胆红素的含量测定方法。方法:本实验采用紫外分光光度法,以药典方法测定胆红素的含量,用重氮化试剂显色,对样品的提取检测波长为533 nm。结果:牛黄至宝丸中胆红素的含量限度为不低于29.8μg/丸,胆红素浓度在0.009 8～0.049 8 mg·mL-1与吸收度呈良好的线性关系(r=0.999 9,n=5),平均回收率为98.74%(RSD 0.45%,n=6)。结论:该法简便、快速、稳定、可靠,可用于测定牛黄至宝丸中胆红素的含量。Objective： To establish a method of determination of bilirution in Niuhuang Zhibao Pills.Method：Bilirubin was determined by UV spectrophotometry according to Pharmacopoeia.The detection wavelength was at 533 nm.Result：The arerage levels of bilirubin in the Niuhuang Zhibao Pills was 29.8 μg one pill.Bilirubin was linear in the range of 0.009 8 ～ 0.049 8 mg.mL-1with r = 0.999 9,（n = 5）.The average recovery（n = 6） was 98.74%（RSD 0.45%）.Conclusion：The methods were proved to be simple sensitive and reproducible.It can be used for the qualitycontrol of Niuhuang Zhibao Pills.

关 键 词：牛黄至宝丸 人工牛黄 胆红素 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]