四逆散及不同配伍干预溃疡性结肠炎的研究  

Study on Sini Powders and Different Compatibilities in Intervening Experimental Ulcerative Colitis

作　　者：卢健[1,2] 范颖[1] 马骥[1] 王丽娜[1] 林蔗茹[1] 徐丹[1] 

机构地区：[1]辽宁中医药大学方剂学科,沈阳110032 [2]辽宁卫生职业技术学院,沈阳110101

出　　处：《中国实验方剂学杂志》2010年第16期109-112,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅项目(2006T093)

摘　　要：目的:研究四逆散及其不同配伍干预实验性溃疡性结肠炎(ulcerative colitis,UC)的效用。方法:将动物分为14组,采用免疫法造模,以大鼠一般状况、结肠组织大体情况、结肠组织病理变化,胸腺指数、脾脏指数和结肠组织核因子-κB(nuclear factor Kappa B,NF-κB)活性作为观察与检测指标。结果:模型组各项指标均与正常组存在显著差异(P<0.01);模型组结肠损伤程度评分为4.00±0.67,与模型组比较,各药组的结肠损伤程度均显著降低(P<0.01);模型组结肠病理组织学评分(4.40±1.07)明显高于正常组(0.40±0.52),P<0.01,余各药组结肠病理组织变化呈好转趋势,其中,四逆散组(2.80±0.79)和柴芍枳组(3.20±0.79)、柴芍甘组(3.40±0.70)、柴芍组(3.20±0.79)、柴枳组效果明显(3.33±0.71),P<0.05;四逆散及其不同配伍能够提高模型大鼠的胸腺指数(P<0.05)与脾脏指数(P<0.01);模型组结肠组织NF-κB平均吸光度为0.088±0.009,各组NF-κB活性均呈现下降趋势,以四逆散全方组(0.023±0.006)效果最为明显(P<0.01)。结论:四逆散能够干预实验性UC,其作用可能是通过刺激免疫器官分泌免疫细胞,抑制NF-κB的激活来实现的;四逆散全方作用最为理想,柴胡、芍药发挥了君药与臣药的的重要配伍作用,甘草作为使药,其协调作用主要体现在对胸腺细胞增殖的刺激。Objective： To study the Sini Powders and the different compatibilities in intervening experimental ulcerative colitis.Method： The experimental rats were divided into 14 groups and modeled with immunization.The status,colon organization and its pathological changes,thymus index,spleen index,activity of nuclear factor-Kappa B（NF-κB） of rats were observed.Result：All parameters of model group showed significant difference with that of the normal group（P 〈 0.01）.Compared with model group（4.40 ± 1.07）,the degrees of colonic injury of all drug groups were significantly lower（P 〈 0.01）.Compared with the model,pathological changes in colon for Sini powders group（2.80 ± 0.79）,Chaishaozhi group（3.20 ± 0.79）,Chaishaogan group（3.40 ± 0.70）,Chaishao group（3.20 ± 0.79） and Chaizhi group（3.33 ± 0.71） were all evident.The Sini Powders and the different compatibilities could improve thymus index and spleen index.And activities of NF-κB of all drug groups were decreased significantly,especially for the Sini Powders（0.023 ± 0.006）（P 〈 0.01）.Conclusion： We speculate that the Sini Powders can intervene with the experimental UC by stimulating the immune organs to excrete the immune cells and inhibiting NF-κB to be activated.Bupleurum and peony,as the monarch drug and theministerial drug in the prescription,play the important roles.Licorice plays a coordinating role through stimulating thymus cells proliferation as the conducting drug.

关 键 词：四逆散 配伍 溃疡性结肠炎 核因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]