益脑康对动脉粥样硬化基础急性缺血性中风大鼠脑组织病理及脑组织血管内皮生长因子表达的影响  

Yinaokang Effect on AS Based AIS SD Mices' Brain VEGF Expression and Brain Pathological Change

作　　者：秦秀德[1] 黄燕[2] 朱磊[1] 周喜燕[1] 陈杰[1] 

机构地区：[1]广州中医药大学,广州510405 [2]广东省中医院,广州510120

出　　处：《中国实验方剂学杂志》2010年第16期166-169,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部十五攻关课题(2004BA721A02)

摘　　要：目的:研究益脑康对动脉粥样硬化(AS)及AS基础上的急性缺血性中风(AIS)SD大鼠脑组织病理及脑组织血管内皮生长因子(VEGF)表达的影响,以期部分阐述其作用机制。方法:将体重(180±20)g SD雄性大鼠115只随机分为A组(正常组)15只;B,C,D,E,F组共100只大鼠(其中C组为AS组、D组为复合模型组、B组为益脑康预防组、E组为益脑康治疗组、F组为立普妥治疗组),第8 d一次性VD3腹腔注射后开始给养高脂饲料复制AS模型,第65 d,除C组外均采用ET-1 MCA附近注射法复制AIS模型。第74 d,每组随机抽取3只检测脑组织病理情况及VEGF表达情况。结果:益脑康组脑组织VEGF免疫组化结果提示血管壁纤维和内皮细胞着色强度为++,强于立普妥组。结论:益脑康可能通过上调AIS大鼠脑组织VEGF表达而发挥对AIS大鼠脑组织的保护作用。Objective：To study how the Yinaokang affect the atheroma（AS） and AS based acute ischemic apoplexy（AIS） SD mices＇ brain VEGF expression and brain pathological changes,so as to explain its mechanism.Method： 115 SD rats weighted（180 ± 20）g were randomly divided into A group（normal） 15;B-F group（model） 100：C is AS group,D is composite group,B is Yi Naokang foregoing group,E is Yinaokang group,F is lipitor group.On the 8th day the B-F group would be given intraperitoneal injection of VD3 one-time and then begain to supply with high fat feed to copy AS model,on the 65th day,inject ET-1 near the MCA area except group C to copy AIS model.On the 74th,3 rats would be picked out randomLy to test their brain VEGF expression and HE stained to observe the brain pathological changes.Result： The VEGF expression in the Yinaokang group is ＋ ＋ while the lipitor group is ＋,the expression of VEGF in the Yinaokang group is stronger than the lipitor group.Conclusion： Yinaokang＇s treatment effect on AIS maybe is related with its function of upregulating VEGF expression in the ischemic brain.

关 键 词：缺血性中风 益脑康 AIS动物模型 VEGF表达 

分 类 号：R285.5[医药卫生—中药学]