金银花叶中绿原酸的大孔吸附树脂纯化工艺研究  

Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin

作　　者：杨军宣[1] 尹蓉莉[1] 陈金玉[1] 吴天骄 王宝才[1] 陈海亭[1] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]四川迪康科技药业股份有限公司,成都610041

出　　处：《中国实验方剂学杂志》2010年第15期20-23,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立金银花叶中绿原酸的含量测定方法,考察不同型号的大孔吸附树脂对金银花叶中绿原酸的提取纯化效果,为金银花药材的综合开发利用提供参考。方法:采用高效液相色谱法测定指标成分。采用静态吸附法和动态吸附法,考察大孔树脂的吸附、洗脱附性能和纯化效果。结果:绿原酸在2.376 8～60.476 9μg与峰面积呈良好线性关系,平均加样回收率96.82%,RSD 1.83%。HPD450大孔吸附树脂纯化效果较好,优化条件为:柱径比3∶1,药液质量浓度0.5～1.0 g·mL-1、吸附速度0.5 BV·h-1、树脂吸附量生药0.3 g·g-1、洗脱溶媒30%乙醇、洗脱速度2 B V·h-1,洗脱溶媒用量2 BV。结论:HPD450大孔树脂对绿原酸纯化效果较好,工艺稳定可行,可用于工业化生产。Objective：To establish a method for determination of chlorogenic acid in Lonicera japonica leaves by HPLC,and study on extracting and purifying chlorogenic acid from Lonicera japonica leaves by different macroporous resin,so as to provide a reference for the comprehensive development and utilization of honeysuckle herbs.Method： The chlorogenic acid was determined with the detection wavelength 327 nm by HPLC.Static adsorption and dynamic adsorption were used to optimize the adsorption and elution process by macroporous resin.Result：Chlorogenic acid was linear in the range of 2.376 8-60.476 9 μg.The average recovery was 96.82%,RSD was 1.83%.At the same time,the purification effect of chlorogenic acid by HPD450 macroporous resin was better than other resins,the optimal conditions were：column diameter ratio 3 ∶ 1,liquid concentration 0.5-1.0 g·mL-1,absorption velocity 0.5 BV·h-1,resin adsorption quantity 0.3 g·g-1,elution solvent 30% ethanol,elution speed 2 BV·h-1,elution solvent volume 2 BV.Conclusion：The process of purification of chlorogenic acid from Lonicera japonica leaves by HPD450 macroporous resin was stable and could be used for industrial production.

关 键 词：金银花叶 绿原酸 大孔吸附树脂 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]