不同采收期大花罗布麻叶中总黄酮的含量测定  

Determination of Flavonoid of Poacyzuan hendersonii Leaves from Different Harvest Time

作　　者：孟庆艳[1,2] 刘圆[3] 孔星芸[1,2] 白红进[1,2] 

机构地区：[1]塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300 [3]西南民族大学少数民族药物研究所,成都610041

出　　处：《中国实验方剂学杂志》2010年第15期50-52,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆生产建设兵团科学技术局政策引导项目(2006YD29);塔里木盆地生物资源保护利用重点实验室开放项目(BR0609)

摘　　要：目的:比较不同采收期大花罗布麻叶中总黄酮的含量并评价出最佳采收期。方法:采用超声法提取大花罗布麻叶中的总黄酮,紫外-可见分光光度法于508 nm下测定。结果:芦丁在22.78～113.88 mg.L-1内呈良好的线性关系(r=0.999 8),平均加样回收率为98.87%,RSD为1.03%(n=9);大花罗布麻叶不同采收期的测定结果为:4～5月份相对较低,6～9月份稳步增长,10月份相对9月份有所降低。结论:本方法简便、准确,重现性好,可用于评价大花罗布麻叶的最佳采收期,为该药材合理开发利用提供科学依据。Objective： Comparing the content of flavonoid in different harvesting periods and to evaluate the optimal harvest time.Method：Extraction of flavonoid with ultrasonic.The content of flavonoid was compared by UV at wavelength of 508 nm.Result：Rutin was linear in the range of 22.78-113.88 mg.L-1（r = 0.999 8） with average recovery of 98.87% and RSD of 1.03%（n = 9）.The measurement results of Poacyzuan hendersonii leaves in different harvesting time：relative low in April and May,unfaltering rose in June to September,little lower in October than in September.Conclusion：The method was simple,accurate,reproducible,it can be used to evaluate the best harvest time of P.hendersonii,and provide a scientific basis for rational development and utilization.

关 键 词：大花罗布麻 总黄酮 不同采收期 紫外-可见分光光度法 

分 类 号：R284.1[医药卫生—中药学]