补肾醒脑方有效部位对脑缺血再灌注大鼠白细胞介素-1β和细胞间黏附分子-1表达的影响  

Effect of Active Fraction of Bushen Xingnao Decoction on Interleukin-1β and Intercellular Adhesion Molecule-1 Expression in Cerebral Ischemia Reperfusion in Rats

作　　者：赵清超[1] 胡久略[1,2] 黄显章[1] 

机构地区：[1]南阳理工学院,河南南阳473004 [2]湖南中医药大学,长沙410007

出　　处：《中国实验方剂学杂志》2010年第15期136-139,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：南阳市科技公关计划项目(2007S1408)

摘　　要：目的:探讨补肾醒脑方有效部位对大脑局灶性脑缺血后再灌注大鼠脑组织白细胞介素-1β(IL-1β)和细胞间黏附分子-1(ICAM-1)表达的作用。方法:采用大鼠大脑中动脉闭塞局灶性脑缺血再灌注模型,观察补肾醒脑方有效部位对脑缺血大鼠神经功能的影响,用免疫组化法检测脑组织IL-1β和ICAM-1的表达。结果:脑缺血2 h再灌注46 h后,模型组与假手术组比较海马及髓质区IL-1β表达阳性细胞数显著增加(P<0.05,P<0.01),海马及皮质区ICAM-1表达阳性细胞数显著增多(P<0.01,P<0.05);补肾醒脑方有效部位生物碱可使髓质区IL-1β阳性细胞数显著减少(P<0.01);苷可使皮质区ICAM-1β阳性细胞数显著减少(P<0.01)。结论:脑缺血再灌注后,脑组织IL-1β和ICAM-1表达增加,补肾醒脑方有效部位能明显改善因缺血所致神经行为功能缺失,生物碱和苷可分别抑制髓质部位IL-1β蛋白表达及皮质区ICAM-1蛋白表达,提示生物碱和苷可能是补肾醒脑方抗脑缺血后炎症反应的部分物质基础。Objective： To investigate the effect of active fraction of Bushen Xingnao Decoction（BSXND） on cerebral IL-1β and ICAM-1 expression in middle cerebral artery occlusion followed reperfusion in rats.Method： The reversible middle cerebral artery occlusion（MCAO） model was established in rats to investigate the role of BSXND in ischemia injury.The scores of neurological deficits were determined by Lorma EZ method in MCAO rats.IL-1β and ICAM-1 expression in cerebral tissue were determined by immunohisto-chemistry.Result： After cerebral ischemia for 2 h followed reperfusion for 46 h,IL-1β positive cells in cerebral hippocampus and medulla increased markedly（P 〈 0.05,P 〈 0.01）,ICAM-1 positive cells in hippocampus and cortex increased remarkably（P 〈 0.01,P 〈 0.05）,alkaloid extracted from BSXND could reduce IL-1β positive cells（P 〈 0.01）,glycoside extracted from BSXND could decrease ICAM-1 positive cells（P 〈 0.01）.Conclusion： After cerebral ischemia reperfusion,both IL-1β and ICAM-1 expression are increased,alkaloid from BSXND could inhibit IL-1β protein expression in cerebral medulla,glycoside from BSXND could inhibit ICAM-1 protein expression in cerebral cortex.Alkaloid and glycosidemay be the partial substance basis of BSXND for antagonizing inflammatory reaction after cerebral ischemia reperfusion.

关 键 词：补肾醒脑方 有效部位 脑缺血-再灌注 神经行为学评分 白介素-1Β 细胞间黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]