更年春方对卵巢切除大鼠海马中脑源性神经营养因子的影响  

Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat

作　　者：赵凡桂[1] 王文君[1] 周文江[2] 李大金[1] 

机构地区：[1]复旦大学附属妇产科医院,上海200011 [2]复旦大学实验动物部,上海200032

出　　处：《中国实验方剂学杂志》2010年第15期140-142,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30472259);上海市卫生局中医药科研基金项目(2004L020A)

摘　　要：目的:研究中药更年春方(GNC)对卵巢切除大鼠海马中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的调节作用。方法:选择10～12月龄SD雌性大鼠随机分为5组(假手术组,模型组,GNC 6.58 g·kg-1组,GNC 3.29 g·kg-1组,尼尔雌醇3.05×10-4 g.kg-1组),切除双侧卵巢建立学习和记忆能力下降的模型,分别用高、低剂量GNC、尼尔雌醇和生理盐水ig 12周,行Morris水迷宫试验,取海马组织,real time PCR法测定海马BDNF mRNA的变化,免疫组化法测定海马CA3区BDNF蛋白的表达。结果:卵巢切除大鼠海马BDNF mRNA和BDNF蛋白水平下降,而高剂量更年春方和尼尔雌醇提高切除卵巢大鼠海马中BDNF mRNA和蛋白的表达。结论:更年春方可以提高卵巢切除大鼠海马中BDNF的表达,可能通过这个机制改善卵巢切除大鼠的学习记忆能力。Objective：To study the effect of Gengnianchun formula（GNC） on BDNF in hippocampus of ovariectomized SD rat.Method： Ten to twelve months old SD rats were randomized into mimic sham operation group,ovariectomized（OVX） group ＋ NS,OVX ＋ high-dose GNC group,OVX ＋ low-dose GNC group and OVX ＋ Nilestriol group.A little fat around ovary was cut in mimic sham operation group rats.Ovariectomy was operated in four other groups.These rats were fed with GNC,nilestriol or NS for 12 weeks respectively.The changes learning and memory were tested by Morris water maze（MWM）.The real time PCR was performed to determine the mRNA level of BDNF in right hippocampuses.And the immunohistochemistry was performed to detect the distribution of protein of BDNF in paraffin section of left hippocampus CA3.Result：Twelve weeks after ovariectomy,the scale of BDNF mRNA and protein decreased in ovariectomized rat,but rats fed with GNC or Nilestriol had higher scale of BDNF mRNA and protein.Conclusion：GNC may increase the BDNF mRNA and protein in hippocampus,and to improve the learning and memory ability for the ovx rats.

关 键 词：更年春方 卵巢切除 海马 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]